Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | extractable activity drastically increases by illuminating cells with white light | Petroselinum crispum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Petroselinum crispum | |
Mn2+ | - |
Petroselinum crispum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
5,7,3'-trihydroxyflavone 7-O-glucoside | - |
Petroselinum crispum | |
0.035 | - |
quercetin | - |
Petroselinum crispum | |
0.046 | - |
5,7,3'-trihydroxyflavone | - |
Petroselinum crispum | |
0.15 | - |
S-adenosyl-L-methionine | with luteolin as substrate | Petroselinum crispum | |
1.2 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one | - |
Petroselinum crispum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Petroselinum crispum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | Petroselinum crispum | luteolin, enzyme directly related to flavone glycoside biosynthesis, biosynthesis of 3'-methoxyflavonoids | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone | chrysoeriol | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | - |
parsley | - |
Purification (Comment) | Organism |
---|---|
partial | Petroselinum crispum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000027 | - |
- |
Petroselinum crispum |
Storage Stability | Organism |
---|---|
-20°C, 1-3 mg protein/ml in 0.02 M Tris-HCl buffer loses about 30% of its activity after 1 month | Petroselinum crispum |
4°C, acetone powder of fresh cells as source of the enzyme can be kept without significant loss of enzyme activity | Petroselinum crispum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isomeric substances isoferulic acid and diosmetin, chrysoeriol, apigenin p-cumaric acid and ferulic acid are not substrates | Petroselinum crispum | ? | - |
? | |
additional information | only ortho-dihydric phenols can function as substrates and only the meta-hydroxyl group is methylated, no reaction takes place if one of the two phenolic hydroxyls is methylated or if only one hydroxyl group is present | Petroselinum crispum | ? | - |
? | |
S-adenosyl-L-methionine + 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one | - |
Petroselinum crispum | ? | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone | eriodictyol | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | luteolin | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone | chrysoeriol | ? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | luteolin, enzyme directly related to flavone glycoside biosynthesis, biosynthesis of 3'-methoxyflavonoids | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone | chrysoeriol | ? | |
S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone | - |
Petroselinum crispum | ? | - |
? | |
S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside | luteolin 7-O-glucoside | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside | chrysoeriol-7-O-glucoside | ? | |
S-adenosyl-L-methionine + quercetin | - |
Petroselinum crispum | S-adenosyl-L-homocysteine + 3'-O-methylquercetin | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | 9.8 | glycine-NaOH buffer with caffeic acid and luteolin as substrates | Petroselinum crispum |
9.7 | - |
- |
Petroselinum crispum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.8 | - |
at higher pH values activity rapidly declines | Petroselinum crispum |