Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tryptamine | activates by 25% | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
gene OsASMT, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged and of nontagged enzyme in Escherichia coli strain BL21(DE3), expression of N- or C-terminally His-tagged enzyme is not successful | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | various amine compounds, including tyramine, tryptamine, N-acetyltryptamine, and 5-methoxytryptamine show no or poor inhibition of the enzyme. The enzyme does not show substrate inhibition at high N-acetylserotonin concentrations | Oryza sativa | |
tryptophan | about 30% inhibition | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.864 | - |
N-acetylserotonin | recombinant enzyme, pH not specified in the publication, 30°C | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Oryza sativa | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | ASMT-specific activity is not altered by the addition of EDTA or the divalent cation Mg2+, suggesting that Oryza sativa ASMT is a cation-independent O-methyltransferase | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N-acetylserotonin | Oryza sativa | - |
S-adenosyl-L-homocysteine + melatonin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cv. Dongjin, gene osASMT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Oryza sativa | - |
shoot | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000007 | - |
purified GST-tagged recombinant enzyme, pH not specified in the publication, 30°C | Oryza sativa |
0.000013 | - |
purified nontagged recombinant enzyme, pH not specified in the publication, 30°C | Oryza sativa |
0.00003 | - |
purified nontagged recombinant enzyme, pH not specified in the publication, 30°C, at 10 mM substrate N-acetylserotonin concentration | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N-acetylserotonin | - |
Oryza sativa | S-adenosyl-L-homocysteine + melatonin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetylserotonin methyltransferase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Oryza sativa |