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Literature summary for 2.1.1.375 extracted from

  • Rahmeh, A.; Jianrong, L.; Kranzusch, P.; Whelan, S.
    Ribose 2'-O methylation of the vesicular stomatitis virus mRNA cap precedes and facilitates subsequent guanine-N-7 methylation by the large polymerase protein (2009), J. Virol., 83, 11043-11050 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.375

Protein Variants

Protein Variants Comment Organism
D1762A mutation inhibits both 2'-O and G-N-7 methylations vesicular stomatitis Indiana virus
E1833Q mutation inhibits both 2'-O and G-N-7 methylations vesicular stomatitis Indiana virus
K1651A mutation inhibits both 2'-O and G-N-7 methylations vesicular stomatitis Indiana virus
K1795A mutation inhibits both 2'-O and G-N-7 methylations vesicular stomatitis Indiana virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] vesicular stomatitis Indiana virus
-
 2 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
2 S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] vesicular stomatitis Indiana virus San Juan
-
 2 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] vesicular stomatitis Indiana virus
-
 S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] vesicular stomatitis Indiana virus San Juan
-
 S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAmACA-[mRNA] vesicular stomatitis Indiana virus
-
 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAmACA-[mRNA] vesicular stomatitis Indiana virus San Juan
-
 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?

Organism

Organism UniProt Comment Textmining
vesicular stomatitis Indiana virus P03523
-
-
vesicular stomatitis Indiana virus San Juan P03523
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + G(5')pppAACA-[mRNA]
-
 vesicular stomatitis Indiana virus 2 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
2 S-adenosyl-L-methionine + G(5')pppAACA-[mRNA]
-
 vesicular stomatitis Indiana virus San Juan 2 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAACA-[mRNA]
-
 vesicular stomatitis Indiana virus S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAACA-[mRNA]
-
 vesicular stomatitis Indiana virus San Juan S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAmACA-[mRNA]
-
 vesicular stomatitis Indiana virus S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?
S-adenosyl-L-methionine + G(5')pppAmACA-[mRNA]
-
 vesicular stomatitis Indiana virus San Juan S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]
-
 ?

Synonyms

Synonyms Comment Organism
RNA-directed RNA polymerase L
-
 vesicular stomatitis Indiana virus
VSV L
-
 vesicular stomatitis Indiana virus

General Information

General Information Comment Organism
physiological function enzyme functions for both 2'-O and G-N-7 methylations during RNA cap formation. VSV L typically modifies the 2'-O position of the cap prior to the G-N-7 position and G-N-7 methylation is diminished by pre-2'-O methylation of the substrate RNA. Amino acid substitutions in the C terminus of L that prevent all cap methylation in recombinant vesicular stomatitis virus partially retain the ability to G-N-7 methylate a pre-2'-O-methylated RNA, uncoupling the effect of substitutions in the C terminus of the L protein on the two methylations. The 2'-O and G-N-7 methylase activities act specifically on RNA substrates that contain the conserved elements of a vesicular stomatitis virus mRNA start at the 5' terminus vesicular stomatitis Indiana virus