Literature summary for 2.1.1.373 extracted from

Curson, A.; Williams, B.; Pinchbeck, B.; Sims, L.; Martinez, A.; Rivera, P.; Kumaresan, D.; Mercade, E.; Spurgin, L.; Carrion, O.; Moxon, S.; Cattolico, R.; Kuzhiumparambil, U.; Guagliardo, P.; Clode, P.; Raina, J.; Todd, J.
DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton (2018), Nat. Microbiol., 3, 430-439 .

Search for more literature for 2.1.1.373

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0601	-	S-adenosyl-L-methionine	22°, pH not specified in the publication	Prymnesium parvum
0.0882	-	(2R)-2-hydroxy-4-(methylsulfanyl)butanoate	22°, pH not specified in the publication	Prymnesium parvum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Prymnesium parvum	9507	-
mitochondrion	-	Prymnesium parvum	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Lingulodinium polyedra	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Symbiodinium microadriaticum	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Prymnesium parvum	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Acropora cervicornis	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Fragilariopsis cylindrus	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Alexandrium tamarense	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Chrysochromulina tobinii	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Chrysochromulina tobinii CCMP291	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Prymnesium parvum CCAP946/1B	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Lingulodinium polyedra CCMP1936	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Alexandrium tamarense CCMP1771	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Prymnesium parvum CCAP941	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Fragilariopsis cylindrus CCMP1102	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Acropora cervicornis CCMP1771	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	Symbiodinium microadriaticum CCMP2467	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Acropora cervicornis	-	-	-
Acropora cervicornis CCMP1771	-	-	-
Alexandrium tamarense	-	-	-
Alexandrium tamarense CCMP1771	-	-	-
Chrysochromulina tobinii	-	-	-
Chrysochromulina tobinii CCMP291	-	-	-
Fragilariopsis cylindrus	-	-	-
Fragilariopsis cylindrus CCMP1102	-	-	-
Lingulodinium polyedra	-	-	-
Lingulodinium polyedra CCMP1936	-	-	-
Prymnesium parvum	-	-	-
Prymnesium parvum CCAP941	-	-	-
Prymnesium parvum CCAP946/1B	-	-	-
Symbiodinium microadriaticum	-	-	-
Symbiodinium microadriaticum CCMP2467	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Prymnesium parvum
-	Fragilariopsis cylindrus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Lingulodinium polyedra	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Symbiodinium microadriaticum	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Prymnesium parvum	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Acropora cervicornis	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Fragilariopsis cylindrus	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Alexandrium tamarense	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Chrysochromulina tobinii	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme shows no detectable methyltransferase activity with other potential substrates including methionine, 4-methylthio-2-oxobutyrate and methylmercaptopropionate	Prymnesium parvum	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Chrysochromulina tobinii CCMP291	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Prymnesium parvum CCAP946/1B	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme shows no detectable methyltransferase activity with other potential substrates including methionine, 4-methylthio-2-oxobutyrate and methylmercaptopropionate	Prymnesium parvum CCAP946/1B	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Lingulodinium polyedra CCMP1936	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Alexandrium tamarense CCMP1771	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Prymnesium parvum CCAP941	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme shows no detectable methyltransferase activity with other potential substrates including methionine, 4-methylthio-2-oxobutyrate and methylmercaptopropionate	Prymnesium parvum CCAP941	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Fragilariopsis cylindrus CCMP1102	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Acropora cervicornis CCMP1771	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	Symbiodinium microadriaticum CCMP2467	S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate	-	?

Synonyms

Synonyms	Comment	Organism
dsyB	-	Lingulodinium polyedra
dsyB	-	Symbiodinium microadriaticum
dsyB	-	Prymnesium parvum
dsyB	-	Acropora cervicornis
dsyB	-	Fragilariopsis cylindrus
dsyB	-	Alexandrium tamarense
dsyB	-	Chrysochromulina tobinii
methylthiohydroxybutryate methyltransferase	-	Lingulodinium polyedra
methylthiohydroxybutryate methyltransferase	-	Symbiodinium microadriaticum
methylthiohydroxybutryate methyltransferase	-	Prymnesium parvum
methylthiohydroxybutryate methyltransferase	-	Acropora cervicornis
methylthiohydroxybutryate methyltransferase	-	Fragilariopsis cylindrus
methylthiohydroxybutryate methyltransferase	-	Alexandrium tamarense
methylthiohydroxybutryate methyltransferase	-	Chrysochromulina tobinii
MMT	-	Lingulodinium polyedra
MMT	-	Symbiodinium microadriaticum
MMT	-	Prymnesium parvum
MMT	-	Acropora cervicornis
MMT	-	Fragilariopsis cylindrus
MMT	-	Alexandrium tamarense
MMT	-	Chrysochromulina tobinii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.499	-	(2R)-2-hydroxy-4-(methylsulfanyl)butanoate	22°C, pH not specified in the publication	Prymnesium parvum
0.515	-	S-adenosyl-L-methionine	22°C, pH not specified in the publication	Prymnesium parvum

Expression

Organism	Comment	Expression
Prymnesium parvum	DSYB transcription levels increases with dimethylsulfoniopropionate concentrations	up

General Information

General Information	Comment	Organism
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Lingulodinium polyedra
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Symbiodinium microadriaticum
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Prymnesium parvum
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Acropora cervicornis
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Fragilariopsis cylindrus
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Alexandrium tamarense
evolution	evolutionary analysis suggests that eukaryotic enzyme (DSYB) originates in bacteria and is passed to eukaryotes early in their evolution	Chrysochromulina tobinii
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Lingulodinium polyedra
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Symbiodinium microadriaticum
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Prymnesium parvum
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Acropora cervicornis
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Fragilariopsis cylindrus
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Alexandrium tamarense
metabolism	the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis in phytoplankton	Chrysochromulina tobinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.6	-	(2R)-2-hydroxy-4-(methylsulfanyl)butanoate	22°C, pH not specified in the publication	Prymnesium parvum
8.5	-	S-adenosyl-L-methionine	22°C, pH not specified in the publication	Prymnesium parvum