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Literature summary for 2.1.1.373 extracted from

  • Kageyama, H.; Tanaka, Y.; Shibata, A.; Waditee-Sirisattha, R.; Takabe, T.
    Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana Identification of a gene encoding MTHB-methyltransferase (2018), Arch. Biochem. Biophys., 645, 100-106 .
    View publication on PubMed
Search for more literature for 2.1.1.373

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) cells Thalassiosira pseudonana

Inhibitors

Inhibitors Comment Organism Structure
KCl inhibits about 50% at concentration of 1.5 M Thalassiosira pseudonana
NaCl inhibits about 50% at concentration of 1.5 M Thalassiosira pseudonana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 S-adenosyl-L-methionine pH 8.0, 25°C Thalassiosira pseudonana
2.1
-
 (2R)-2-hydroxy-4-(methylsulfanyl)butanoate pH 8.0, 25°C Thalassiosira pseudonana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Thalassiosira pseudonana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate Thalassiosira pseudonana the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis. Dimethylsulfoniopropionate plays a pivotal role in the marine sulfur cycle S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate Thalassiosira pseudonana CCMP1335 the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis. Dimethylsulfoniopropionate plays a pivotal role in the marine sulfur cycle S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Thalassiosira pseudonana B8C5X1
-
-
Thalassiosira pseudonana CCMP1335 B8C5X1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thalassiosira pseudonana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis. Dimethylsulfoniopropionate plays a pivotal role in the marine sulfur cycle Thalassiosira pseudonana S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate
-
 Thalassiosira pseudonana CCMP1335 S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?
S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis. Dimethylsulfoniopropionate plays a pivotal role in the marine sulfur cycle Thalassiosira pseudonana CCMP1335 S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Thalassiosira pseudonana

Synonyms

Synonyms Comment Organism
MTHB-methyltransferase
-
 Thalassiosira pseudonana
Tp23128
-
 Thalassiosira pseudonana
TpMT2
-
 Thalassiosira pseudonana
XP_002291473
-
 Thalassiosira pseudonana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Thalassiosira pseudonana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thalassiosira pseudonana

Expression

Organism Comment Expression
Thalassiosira pseudonana high salinity and nitrogen limitation stresses cause the increase of dimethylsulfoniopropionate content and TpMMT protein in Thalassiosira up

General Information

General Information Comment Organism
metabolism the enzyme catalyses the key step of dimethylsulfoniopropionate biosynthesis. Dimethylsulfoniopropionate plays a pivotal role in the marine sulfur cycle Thalassiosira pseudonana