Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
A34V/K44Q/M66T/L214S/Y229H | isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites | Salmonella enterica |
L214S/Y229H | isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites | Salmonella enterica |
N172S | isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites, the mutant enzyme displays enhanced protection of the cell DNA against the Sau96I endonuclease. The protection is not sufficient to support long-term survival in the presence of the inducer, which is consistent with incomplete methylation of GG(G/C)CC sites in plasmid DNA purified from the mutant | Salmonella enterica |
V173L | isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites, the mutant enzyme displays enhanced protection of the cell DNA against the Sau96I endonuclease. The protection is not sufficient to support long-term survival in the presence of the inducer, which is consistent with incomplete methylation of GG(G/C)CC sites in plasmid DNA purified from the mutant | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Salmonella enterica | SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar Infantis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine | Salmonella enterica | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine, molecular mechanism of substrate recognition, overview | Salmonella enterica | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SinI DNA methyltransferase | - |
Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Salmonella enterica |