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Literature summary for 2.1.1.37 extracted from

  • van Bemmel, D.M.; Brank, A.S.; Eritja, R.; Marquez, V.E.; Christman, J.K.
    DNA (Cytosine-C5) methyltransferase inhibition by oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone (zebularine aglycon) at the enzymatic target site (2009), Biochem. Pharmacol., 78, 633-641.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
M.HhaI overexpression in Escherichia coli strain ER1727 Haemophilus parahaemolyticus

Inhibitors

Inhibitors Comment Organism Structure
2-(1H)-pyrimidinone riboside i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus
5-azacytidine zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds Haemophilus parahaemolyticus
additional information inhibitor binding kinetics Haemophilus parahaemolyticus
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview Haemophilus parahaemolyticus
oligodeoxyribonucleotides containing 5-azacytidine 5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview Haemophilus parahaemolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Haemophilus parahaemolyticus
-
 DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Haemophilus parahaemolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine
-
 Haemophilus parahaemolyticus DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
DNA (cytosine-C5) methyltransferase
-
 Haemophilus parahaemolyticus
M.HhaI
-
 Haemophilus parahaemolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Haemophilus parahaemolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Haemophilus parahaemolyticus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine the ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus