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Literature summary for 2.1.1.37 extracted from

  • Suetake, I.; Miyazaki, J.; Murakami, C.; Takeshima, H.; Tajima, S.
    Distinct enzymatic properties of recombinant mouse DNA methyltransferases Dnmt3a and Dnmt3b (2003), J. Biochem., 133, 737-744.
    View publication on PubMed
Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cell Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
K+ Dnmt3b Mus musculus
Mg2+ 1 mM, slight inhibition, Dnmt3a; 1 mM, slight inhibition, Dnmt3b Mus musculus
Mn2+ strong inhibition, Dnmt3a; strong inhibition, Dnmt3b Mus musculus
additional information Dnmt3a is fully active at physiological potassium concentration of K+ Mus musculus
NaCl Dnmt3a, inhibits activity around physiological ionic strength; Dnmt3b, inhibits activity around physiological ionic strength Mus musculus
Ni2+ strong inhibition, Dnmt3a; strong inhibition, Dnmt3b Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 S-adenosyl-L-methionine pH 7.4, 37°C, Dnmt3a Mus musculus
0.0004
-
 dIdC pH 7.4, 37°C, Dnmt3b Mus musculus
0.0005
-
 dGdC pH 7.4, 37°C, Dnmt3b Mus musculus
0.0007
-
 S-adenosyl-L-methionine pH 7.4, 37°C, Dnmt3b Mus musculus
0.0012
-
 dIdC pH 7.4, 37°C, Dnmt3b Mus musculus
0.0016
-
 dGdC pH 7.4, 37°C, Dnmt3b Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA Mus musculus the enzyme is a de novo-type DNA methyltransferase S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O88508 Dnmt3a
-
Mus musculus O88509 Dnmt3b
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with CpT and CpC Mus musculus ?
-
 ?
additional information scarcely methylates CpC Mus musculus ?
-
 ?
S-adenosyl-L-methionine + CpA
-
 Mus musculus S-adenosyl-L-homocysteine + CpA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + CpG
-
 Mus musculus S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + CpT
-
 Mus musculus S-adenosyl-L-homocysteine + CpT containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + dGdC
-
 Mus musculus ?
-
 ?
S-adenosyl-L-methionine + dIdC
-
 Mus musculus ?
-
 ?
S-adenosyl-L-methionine + DNA the enzyme is a de novo-type DNA methyltransferase Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + DNA poly(dGdC)-poly(dGdC) or poly(dIdC)-poly(dIdC) Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Synonyms

Synonyms Comment Organism
Dnmt3a
-
 Mus musculus
DNMT3B
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.8 Dnmt3b Mus musculus
7 7.8 Dnmt3a Mus musculus