Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Bacillus stearothermophilus, expressed as N-terminal fusion at ATC149-151 or ATG344-345 to histidine taggs | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, isoform II, SDS-PAGE | Geobacillus stearothermophilus |
66000 | - |
x * 66000, isoform I, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q60171 | enzyme M.BssHII expressed as two isoforms: isoform I and isoform II | - |
Purification (Comment) | Organism |
---|---|
expressed as N-terminal fusion at ATC149-151 or ATG344-345 to histidine taggs | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA | - |
Geobacillus stearothermophilus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, isoform II, SDS-PAGE | Geobacillus stearothermophilus |
? | x * 66000, isoform I, SDS-PAGE | Geobacillus stearothermophilus |