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Literature summary for 2.1.1.37 extracted from

  • Sethmann, S.; Ceglowski, P.; Willert, J.; Iwanicka-Nowicka, R.; Trautner, T.A.; Walter, J.
    M.(f)BssHII, a novel cytosine-C5-DNA-methyltransferase with target-recognizing domains at separated locations of the enzyme (1999), EMBO J., 18, 3502-3508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Bacillus stearothermophilus, expressed as N-terminal fusion at ATC149-151 or ATG344-345 to histidine taggs Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, isoform II, SDS-PAGE Geobacillus stearothermophilus
66000
-
x * 66000, isoform I, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q60171 enzyme M.BssHII expressed as two isoforms: isoform I and isoform II
-

Purification (Commentary)

Purification (Comment) Organism
expressed as N-terminal fusion at ATC149-151 or ATG344-345 to histidine taggs Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
Geobacillus stearothermophilus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?

Subunits

Subunits Comment Organism
? x * 59000, isoform II, SDS-PAGE Geobacillus stearothermophilus
? x * 66000, isoform I, SDS-PAGE Geobacillus stearothermophilus