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Literature summary for 2.1.1.361 extracted from
- Multivalent interactions by the Set8 histone methyltransferase with its nucleosome substrate (2016), J. Mol. Biol., 428, 1531-1543 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R179A/K180A | the mutations lead to similar enzymatic activity as the wild type equivalent and about 2.5fold decrease in nucleosome binding affinity | Homo sapiens |
| R179A/K180A/R188A/R189A | the mutations produce similar enzymatic activity and even weaker nucleosome binding as compared to the R188A/R189A mutant | Homo sapiens |
| R188A/R189A | the mutant has reduced nucleosome methyltransferase and much lower nucleosome binding activity (56% of full length, wild type enzyme activity, and 0.0025 mM nucleosome binding dissociation constant or over 300fold lower binding affinity compared to the equivalent wild type protein) | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme binding to the nucleosome protects it from degradation during the cell cycle. Full length enzyme has 3.8% of its histone methyltransferase activity on nucleosomes containing the H2A/E61A/ E64A/D90A/E92A mutations compared to wild type nucleosomes | ? | - |
? |  |
| S-adenosyl-L-methionine + a [histone H4]-L-lysine20 | Homo sapiens | - |
S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme binding to the nucleosome protects it from degradation during the cell cycle. Full length enzyme has 3.8% of its histone methyltransferase activity on nucleosomes containing the H2A/E61A/ E64A/D90A/E92A mutations compared to wild type nucleosomes | Homo sapiens | ? | - |
? | |
| S-adenosyl-L-methionine + a [histone H4]-L-lysine20 | - |
Homo sapiens | S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SET8 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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