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Literature summary for 2.1.1.361 extracted from

  • Kudithipudi, S.; Dhayalan, A.; Kebede, A.F.; Jeltsch, A.
    The SET8 H4K20 protein lysine methyltransferase has a long recognition sequence covering seven amino acid residues (2012), Biochimie, 94, 2212-2218.
    View publication on PubMed
Search for more literature for 2.1.1.361

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, GST-tagged SET8 domain, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information In addition to K20 (the target methylation site), R17 and H18 are very important specificity determinants for SET8 for substrate recognition. Any other amino acid introduced at these positions completely abolishes the activity of SET8. SET8 equally accepts K and R at position 19, followed by Y and other hydrophobic amino acids like L, H and I. Residues on the C-terminal side of K20 also play important roles in the specificity of SET8. The enzyme accepts majorly I at the -1 position followed by V. Other hydrophobic amino acids like F, Y and L give weaker methylation signals. SET8 also exhibits strong specificity on L22 and R23. At L22 some other hydrophobic amino acids are tolerated. At the +3 position, SET8 majorly prefers R, however it exhibits weak methylation when itis exchanged with other amino acids. SET8 activity is strongly reduced by the arginine methylation at positions R17 in case of H4 and R379 in case of p53 Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + histone H4(K20) SET8 specifically methylates K20 on the histone H4. Enzyme activity is strongly reduced by the arginine methylation at position R17 Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + p53 (K382) SET8 activity is strongly reduced by the arginine methylation at position R379 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, GST-tagged SET8 domain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
H4K20 protein lysine methyltransferase
-
 Homo sapiens
KMT5a
-
 Homo sapiens
PKMT
-
 Homo sapiens
Pr-Set7
-
 Homo sapiens
protein lysine methyltransferase
-
 Homo sapiens
SET8
-
 Homo sapiens