Literature summary for 2.1.1.360 extracted from

Jang, S.; Kang, C.; Yang, H.S.; Jung, T.; Hebert, H.; Chung, K.Y.; Kim, S.J.; Hohng, S.; Song, J.J.
Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase (2019), Genes Dev., 33, 620-625 .

Search for more literature for 2.1.1.360

Activating Compound

Activating Compound	Comment	Organism	Structure
histone H2B ubiquitin	the enzyme activity is stimulated by histone H2B Lys120 ubiquitination	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F326A	the mutant shows significantly decreased enzyme activity compared to the wild type	Homo sapiens
I290A	the mutant shows significantly decreased enzyme activity compared to the wild type	Homo sapiens
L284A	the mutant shows significantly decreased enzyme activity compared to the wild type	Homo sapiens
R282A	the mutant shows dramatically decreased enzyme activity compared to the wild type	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine79	Homo sapiens	overall reaction	3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine79	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8TEK3	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutahione S-transferase affinity column chromatography, cation exchange chromatography and S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine79	overall reaction	Homo sapiens	3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine79	-	?

Synonyms

Synonyms	Comment	Organism
DOT1L	-	Homo sapiens
histone H3 Lys79 methyltransferase	-	Homo sapiens

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Release 2023.1 (January 2023)