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Literature summary for 2.1.1.359 extracted from

  • Hacker, K.E.; Fahey, C.C.; Shinsky, S.A.; Chiang, Y.J.; DiFiore, J.V.; Jha, D.K.; Vo, A.H.; Shavit, J.A.; Davis, I.J.; Strahl, B.D.; Rathmell, W.K.
    Structure/function analysis of recurrent mutations in SETD2 protein reveals a critical and conserved role for a SET domain residue in maintaining protein stability and histone H3 Lys-36 trimethylation (2016), J. Biol. Chem., 291, 21283-21295 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.359

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R1625C the mutation results in a complete loss of histone H3 lysine 36 trimethylation activity. The mutant shows unchanged thermal stability as compared with the wild type protein but diminished binding to the histone H3 tail Homo sapiens
R195C the mutation results in a complete loss of histone H3 lysine 36 trimethylation activity but does not affect dimethylated histone H3 lysine 36 Saccharomyces cerevisiae
R2510H the mutation does not result in an observable defect in enzymatic function Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 Saccharomyces cerevisiae overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine36 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine36 Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine36 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine36 Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine36 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine36 Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BYW2
-
-
Saccharomyces cerevisiae P46995
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 overall reaction Saccharomyces cerevisiae 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine36
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine36
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine36
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine36
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?

Synonyms

Synonyms Comment Organism
SET2
-
 Saccharomyces cerevisiae
SETD2
-
 Homo sapiens