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Literature summary for 2.1.1.356 extracted from

  • Jiao, L.; Liu, X.
    Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2 (2015), Science, 350, aac4383 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.356

Activating Compound

Activating Compound Comment Organism Structure
[histone H3]-N6,N6,N6-trimethyl-L-lysine27 the basal enzyme activity is stimulated by [histone H3]-N6,N6,N6-trimethyl-L-lysine27 Thermochaetoides thermophila

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain CB010 Thermochaetoides thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 15% (w/v) polyethylene glycol 4000 and 175 mM ammonium citrate (pH 7.0) Thermochaetoides thermophila

Protein Variants

Protein Variants Comment Organism
D329A the mutant shows strongly reduced activity compared to the wild type enzyme Thermochaetoides thermophila
DELTAL350 the mutant shows strongly reduced activity compared to the wild type enzyme Thermochaetoides thermophila
H326A the mutant shows strongly reduced activity compared to the wild type enzyme Thermochaetoides thermophila
P325S the mutant shows strongly reduced activity compared to the wild type enzyme Thermochaetoides thermophila
R839D the mutant shows strongly reduced activity compared to the wild type enzyme Thermochaetoides thermophila

Inhibitors

Inhibitors Comment Organism Structure
histone H3 mutant K27M
-
 Thermochaetoides thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Thermochaetoides thermophila overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Thermochaetoides thermophila DSM1495 overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine27 Thermochaetoides thermophila
-
 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine27 Thermochaetoides thermophila DSM1495
-
 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine27 Thermochaetoides thermophila
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine27 Thermochaetoides thermophila DSM1495
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27 Thermochaetoides thermophila
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27 Thermochaetoides thermophila DSM1495
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-
Thermochaetoides thermophila DSM1495
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin resin column chromatography and Superdex S200 gel filtration Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 overall reaction Thermochaetoides thermophila 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 overall reaction Thermochaetoides thermophila DSM1495 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
 Thermochaetoides thermophila S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
 Thermochaetoides thermophila DSM1495 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 Thermochaetoides thermophila S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 Thermochaetoides thermophila DSM1495 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27
-
 Thermochaetoides thermophila S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27
-
 Thermochaetoides thermophila DSM1495 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Subunits

Subunits Comment Organism
? x * 170000, SDS-PAGE Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
EZH2 catalytic domain Thermochaetoides thermophila
Polycomb repressive complex 2
-
 Thermochaetoides thermophila
PRC2
-
 Thermochaetoides thermophila