Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paramecium bursaria Chlorella virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
L116A | the mutant shows strongly increased activity compared to the wild type enzyme | Paramecium bursaria Chlorella virus 1 |
Y109G | the mutant shows strongly decreased activity compared to the wild type enzyme | Paramecium bursaria Chlorella virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.062 | - |
S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.072 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.086 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.086 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.126 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.128 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.137 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.173 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.917 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
2.8 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 | Paramecium bursaria Chlorella virus 1 | overall reaction | 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-L-lysine27 | Paramecium bursaria Chlorella virus 1 | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 | Paramecium bursaria Chlorella virus 1 | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 | Paramecium bursaria Chlorella virus 1 | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus 1 | O41094 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin column chromatography and Superdex 75 gel filtration | Paramecium bursaria Chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
(+/-)-2'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
(+/-)-3'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 | overall reaction | Paramecium bursaria Chlorella virus 1 | 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 | - |
Paramecium bursaria Chlorella virus 1 | S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EZH2 | - |
Paramecium bursaria Chlorella virus 1 |
histone H3 lysine 27 methyltransferase | - |
Paramecium bursaria Chlorella virus 1 |
Polycomb repressive complex 2 | - |
Paramecium bursaria Chlorella virus 1 |
PRC2 | - |
Paramecium bursaria Chlorella virus 1 |
SET | - |
Paramecium bursaria Chlorella virus 1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000008 | - |
S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.000008 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.00004 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.002 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.002 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.002 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0025 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.006 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0217 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.065 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.123 | - |
S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000003 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.000043 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.000128 | - |
S-adenosyl-L-methionine | mutant enzyme Y109G, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0156 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0183 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0252 | - |
(+/-)-2'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.027 | - |
(+/-)-3'-benzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.0357 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.3 | - |
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
0.516 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 | |
2.056 | - |
S-adenosyl-L-methionine | mutant enzyme L116A, at pH 8.0 and 23°C | Paramecium bursaria Chlorella virus 1 |