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Literature summary for 2.1.1.356 extracted from

  • Li, J.; Wei, H.; Zhou, M.M.
    Structure-guided design of a methyl donor cofactor that controls a viral histone H3 lysine 27 methyltransferase activity (2011), J. Med. Chem., 54, 7734-7738 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paramecium bursaria Chlorella virus 1

Protein Variants

Protein Variants Comment Organism
L116A the mutant shows strongly increased activity compared to the wild type enzyme Paramecium bursaria Chlorella virus 1
Y109G the mutant shows strongly decreased activity compared to the wild type enzyme Paramecium bursaria Chlorella virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.062
-
S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.072
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.086
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.086
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.126
-
S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.128
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.137
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.173
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.917
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.8
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Paramecium bursaria Chlorella virus 1 overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Paramecium bursaria Chlorella virus 1
-
S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 Paramecium bursaria Chlorella virus 1
-
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 Paramecium bursaria Chlorella virus 1
-
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
?

Organism

Organism UniProt Comment Textmining
Paramecium bursaria Chlorella virus 1 O41094
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin column chromatography and Superdex 75 gel filtration Paramecium bursaria Chlorella virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
Paramecium bursaria Chlorella virus 1 ?
-
?
(+/-)-2'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
Paramecium bursaria Chlorella virus 1 ?
-
?
(+/-)-3'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
Paramecium bursaria Chlorella virus 1 ?
-
?
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 overall reaction Paramecium bursaria Chlorella virus 1 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-L-lysine27
-
Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27
-
Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
?

Synonyms

Synonyms Comment Organism
EZH2
-
Paramecium bursaria Chlorella virus 1
histone H3 lysine 27 methyltransferase
-
Paramecium bursaria Chlorella virus 1
Polycomb repressive complex 2
-
Paramecium bursaria Chlorella virus 1
PRC2
-
Paramecium bursaria Chlorella virus 1
SET
-
Paramecium bursaria Chlorella virus 1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000008
-
S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.000008
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.00004
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.002
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.002
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.002
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0025
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.006
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0217
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.065
-
S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.123
-
S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000003
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.000043
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.000128
-
S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0156
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0183
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0252
-
(+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.027
-
(+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.0357
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.3
-
(+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
0.516
-
S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.056
-
S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1