Application | Comment | Organism |
---|---|---|
analysis | three-dimensional solution structure of vSET bound to S-adenosyl-L-homocysteine and a histone H3 peptide containing mono-methylated lysine 27, only invariant active site residue tyrosine 105 in vSET facilitates methyl transfer from S-adenosyl-L-homocysteine to the substrate lysine by aligning intermolecular interactions in the lysine access channel of the enzyme | Paramecium bursaria Chlorella virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
F52Y | can only mono- and di- but not tri-methylate H38(K27) | Paramecium bursaria Chlorella virus 1 |
Y50F | nearly abolishes di- and tri-methylation of H3(K27), but does not affect the mono-methylation | Paramecium bursaria Chlorella virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2762 | - |
histone H3(K27) | wild-type | Paramecium bursaria Chlorella virus 1 | |
0.4274 | - |
histone H3(K27) | mutant Y50F | Paramecium bursaria Chlorella virus 1 | |
3.9 | - |
histone H3(K27) | mutant F52Y | Paramecium bursaria Chlorella virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + histone H3(K27) | - |
Paramecium bursaria Chlorella virus 1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution | Paramecium bursaria Chlorella virus 1 |
Synonyms | Comment | Organism |
---|---|---|
histone H3 lysine 27 methyltransferase | - |
Paramecium bursaria Chlorella virus 1 |
SET domain histone lysine methyltransferase | - |
Paramecium bursaria Chlorella virus 1 |
vSET | - |
Paramecium bursaria Chlorella virus 1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
histone H3(K27) | wild-type | Paramecium bursaria Chlorella virus 1 | |
1.36 | - |
histone H3(K27) | mutant Y50F | Paramecium bursaria Chlorella virus 1 |