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Literature summary for 2.1.1.356 extracted from

  • Qian, C.; Wang, X.; Manzur, K.; Sachchidanand, K.; Farooq, A.; Zeng, L.; Wang, R.; Zhou, M.M.
    Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase (2006), J. Mol. Biol., 359, 86-96.
    View publication on PubMed

Application

Application Comment Organism
analysis three-dimensional solution structure of vSET bound to S-adenosyl-L-homocysteine and a histone H3 peptide containing mono-methylated lysine 27, only invariant active site residue tyrosine 105 in vSET facilitates methyl transfer from S-adenosyl-L-homocysteine to the substrate lysine by aligning intermolecular interactions in the lysine access channel of the enzyme Paramecium bursaria Chlorella virus 1

Protein Variants

Protein Variants Comment Organism
F52Y can only mono- and di- but not tri-methylate H38(K27) Paramecium bursaria Chlorella virus 1
Y50F nearly abolishes di- and tri-methylation of H3(K27), but does not affect the mono-methylation Paramecium bursaria Chlorella virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2762
-
histone H3(K27) wild-type Paramecium bursaria Chlorella virus 1
0.4274
-
histone H3(K27) mutant Y50F Paramecium bursaria Chlorella virus 1
3.9
-
histone H3(K27) mutant F52Y Paramecium bursaria Chlorella virus 1

Organism

Organism UniProt Comment Textmining
Paramecium bursaria Chlorella virus 1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + histone H3(K27)
-
Paramecium bursaria Chlorella virus 1 ?
-
?

Subunits

Subunits Comment Organism
dimer in solution Paramecium bursaria Chlorella virus 1

Synonyms

Synonyms Comment Organism
histone H3 lysine 27 methyltransferase
-
Paramecium bursaria Chlorella virus 1
SET domain histone lysine methyltransferase
-
Paramecium bursaria Chlorella virus 1
vSET
-
Paramecium bursaria Chlorella virus 1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.32
-
histone H3(K27) wild-type Paramecium bursaria Chlorella virus 1
1.36
-
histone H3(K27) mutant Y50F Paramecium bursaria Chlorella virus 1