Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA | the presence of RNA strongly inhibits enzyme activity in vitro | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | Schizosaccharomyces pombe | overall reaction | 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-L-lysine9 | Schizosaccharomyces pombe | - |
S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9 | Schizosaccharomyces pombe | - |
S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9 | Schizosaccharomyces pombe | - |
S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex S200 gel filtration | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | overall reaction | Schizosaccharomyces pombe | 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-L-lysine9 | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9 | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55920, calculated from amino acid sequence | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Clr4 | - |
Schizosaccharomyces pombe |
H3K9 methyltransferase | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme contributes to heterochromatin formation | Schizosaccharomyces pombe |