Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is stimulated by histone H2B Lys120 ubiquitination | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F326A | the mutant shows significantly reduced activity compared to the wild type enzyme | Homo sapiens |
F326A | the mutation slightly decreases the enzyme activity | Homo sapiens |
I290A | the mutant shows significantly reduced activity compared to the wild type enzyme | Homo sapiens |
L284A | the mutant shows significantly reduced activity compared to the wild type enzyme | Homo sapiens |
L329A | the mutant shows significantly reduced activity compared to the wild type enzyme | Homo sapiens |
R282A | the mutation dramatically decreases the enzyme activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | Homo sapiens | overall reaction | 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | Homo sapiens | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 | Homo sapiens | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9 | Homo sapiens | - |
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + H2B ubiquitinated nucleosome | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TEK3 | - |
- |
Purification (Comment) | Organism |
---|---|
glutahione S-transferase affinity column chromatography, anion exchange column chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | overall reaction | Homo sapiens | 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + H2B ubiquitinated nucleosome | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DOT1L | - |
Homo sapiens |
histone H3 Lys79 methyltransferase | - |
Homo sapiens |