Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain XL1 blue | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
D209A | in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5 | Neurospora crassa |
D209S | in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5 | Neurospora crassa |
E227A | in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5 | Neurospora crassa |
E227S | in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5 | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | 0.0013 | S-adenosyl-L-methionine | in methylation buffer containing 50 mM glycine (pH 9.8), 2 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 10% (v/v) glycerol, at 22°C | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + histone H3(K9) | Dim-5 recognizes R8-G12 of the H3 tail with T11 and G12 being the most important specificity determinants, phosphorylation of H3 tail residues S10 and T11may regulate the activity of Dim-5 | Neurospora crassa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dim-5 | - |
Neurospora crassa |
Dim-5 histone-3 lysine-9 methyltransferase | - |
Neurospora crassa |
HKMT | - |
Neurospora crassa |