Literature summary for 2.1.1.355 extracted from

Gowher, H.; Zhang, X.; Cheng, X.; Jeltsch, A.
Avidin plate assay system for enzymatic characterization of a histone lysine methyltransferase (2005), Anal. Biochem., 342, 287-291.

Search for more literature for 2.1.1.355

Application

Application	Comment	Organism
analysis	development of a microplate biotin/avidin peptide methylation assay, which is convenient, very accurate, reproducible, and inexpensive. Because it yields quantitative results, it can be employed for a characterization of the enzymatic properties of histone lysine methyltransferases and other protein methyltransferases and is also well suited for high-throughput applications	Neurospora crassa

Protein Variants

Protein Variants	Comment	Organism
F281W	shows catalytic activity	Neurospora crassa
F281Y	shows catalytic activity	Neurospora crassa
Y178F	shows catalytic activity, although the methylation rates of this variant are low	Neurospora crassa
Y178V	shows catalytic activity	Neurospora crassa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0074	-	biotinyl-MARTKQTARKSTGGKAPRKQ	-	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purity greater than 95%	Neurospora crassa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
biotinyl-MARTKQTARKSTGGKAPRKQ + S-adenosyl-L-methionine	Dim-5 methylates the histone H3 tail at position Lys9	Neurospora crassa	?	-	?

Synonyms

Synonyms	Comment	Organism
Dim-5	-	Neurospora crassa
Dim-5 histone lysine methyltransferase	-	Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.038	-	biotinyl-MARTKQTARKSTGGKAPRKQ	-	Neurospora crassa

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Release 2023.1 (January 2023)