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Literature summary for 2.1.1.354 extracted from

  • Blazer, L.L.; Lima-Fernandes, E.; Gibson, E.; Eram, M.S.; Loppnau, P.; Arrowsmith, C.H.; Schapira, M.; Vedadi, M.
    PR domain-containing protein 7 (PRDM7) is a histone 3 lysine 4 trimethyltransferase (2016), J. Biol. Chem., 291, 13509-13519 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.354

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S289N the PRDM7 mutant loses the dimethylated substrate specificity and instead gains the ability to methylate H3K36 similar to, but less efficiently than its evolutionary predecessor PRDM9 Homo sapiens
S289N/S312W/S357Y the PRDM7 mutant regains the substrate specificities and catalytic activities similar to its evolutionary predecessor PRDM9, including the ability to efficiently methylate histone H3 lysine 36 Homo sapiens
S312W the PRDM7 mutant loses the dimethylated substrate specificity and instead gains the ability to methylate H3K36 similar to, but less efficiently than its evolutionary predecessor PRDM9 Homo sapiens
S357Y the PRDM7 mutant regains the substrate specificities and catalytic activities similar to its evolutionary predecessor PRDM9, including the ability to efficiently methylate histone H3 lysine 36 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
0.0002
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.00026
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
0.0003
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
0.0004
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.0004
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0007
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.0008
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.001
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0017
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
0.002
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
0.002
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0033
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
0.0035
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.004
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.014
-
 S-adenosyl-L-methionine mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
0.025
-
 S-adenosyl-L-methionine mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
0.056
-
 S-adenosyl-L-methionine mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.2
-
 S-adenosyl-L-methionine mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.9
-
 S-adenosyl-L-methionine wild type enzyme, at pH 9.0 and 23°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine4 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine4 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine4 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine4 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine4
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NQW5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine4 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine4
-
 ?
additional information no activity with ARTAQTARKSTGGKAPRKQLATKAA-GK-biotin, ATKAARKSAPATGGVKKPHRYRPG-GK-biotin, ATKAARKSAPATGGV(Kme1)KPHRYRPGGK-biotin, and ATKAARKSAPATGGV(Kme2)KPHRYRPG-GK-biotin Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine4
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine4
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine4
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine4
-
 ?
S-adenosyl-L-methionine + ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin
-
 Homo sapiens S-adenosyl-L-homocysteine + ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin
-
 ?
S-adenosyl-L-methionine + ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin highest activity Homo sapiens S-adenosyl-L-homocysteine + ART(Kme3)QTARKSTGGKAPRKQLATKAA-GK-biotin
-
 ?
S-adenosyl-L-methionine + ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin
-
 Homo sapiens S-adenosyl-L-homocysteine + ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin
-
 ?

Synonyms

Synonyms Comment Organism
histone 3 lysine 4 trimethyltransferase
-
 Homo sapiens
PR domain-containing protein 7
-
 Homo sapiens
PRDM7
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0022
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.0025
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.0031
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.0036
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.0139
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0167
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0186
-
 S-adenosyl-L-methionine mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.0194
-
 S-adenosyl-L-methionine mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.021
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N, at pH 9.0 and 23°C Homo sapiens
0.0333
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S312W, at pH 9.0 and 23°C Homo sapiens
0.053
-
 S-adenosyl-L-methionine wild type enzyme, at pH 9.0 and 23°C Homo sapiens
0.053
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin wild type enzyme, at pH 9.0 and 23°C Homo sapiens
3.056
-
 S-adenosyl-L-methionine mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
4.167
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
4.806
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
5.583
-
 S-adenosyl-L-methionine mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
5.833
-
 ARTKQTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens
6
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
6.444
-
 ART(Kme2)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S357Y, at pH 9.0 and 23°C Homo sapiens
7.5
-
 ART(Kme1)QTARKSTGGKAPRKQLATKAA-GK-biotin mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C Homo sapiens