Literature summary for 2.1.1.35 extracted from

Urbonavicius, J.; Auxilien, S.; Walbott, H.; Trachana, K.; Golinelli-Pimpaneau, B.; Brochier-Armanet, C.; Grosjean, H.
Acquisition of a bacterial RumA-type tRNA(uracil-54, C5)-methyltransferase by Archaea through an ancient horizontal gene transfer (2008), Mol. Microbiol., 67, 323-335.

Search for more literature for 2.1.1.35

Cloned(Commentary)

Cloned (Comment)	Organism
genes PAB0719 and PAB0760, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)	Pyrococcus abyssi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Pyrococcus abyssi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains an [Fe4S4] cluster, overview	Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54	Pyrococcus abyssi	-	S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	-	genes PAB0719 and PAB0760	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54	-	Pyrococcus abyssi	S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54	-	?
S-adenosyl-L-methionine + tRNA containing uridine at position 54	PAB0719 enzyme or PabTrmU54 displays an identical specificity to eucaryontal homologue TrmA, as it catalyses the in vitro formation of m5U-54 in tRNA	Pyrococcus abyssi	S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54	-	?

Synonyms

Synonyms	Comment	Organism
PAB0719 enzyme	-	Pyrococcus abyssi
PabTrmU54	-	Pyrococcus abyssi
RumA	-	Pyrococcus abyssi
RumA-type tRNA(uracil-54, C5)-methyltransferase	-	Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Pyrococcus abyssi

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	dependent on	Pyrococcus abyssi

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Release 2023.1 (January 2023)