Application | Comment | Organism |
---|---|---|
molecular biology | enzyme is used for detecting tRNA-like moieties in viral RNA | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | at 0.002 mM 50% inhibition, E. coli MRE 600 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
MRE 600 | - |
Escherichia coli MRE 600 | - |
MRE 600 | - |
Purification (Comment) | Organism |
---|---|
affinity elution and hydroxylapatite chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00134 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, at least 6 months, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Escherichia coli | additional information | - |
? | |
additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Bacillus subtilis | additional information | - |
? | |
additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Enterococcus faecalis | additional information | - |
? | |
additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Bacillus cereus | additional information | - |
? | |
additional information | - |
Escherichia coli MRE 600 | additional information | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |