Literature summary for 2.1.1.348 extracted from

Wang, X.; Feng, J.; Xue, Y.; Guan, Z.; Zhang, D.; Liu, Z.; Gong, Z.; Wang, Q.; Huang, J.; Tang, C.; Zou, T.; Yin, P.
Corrigendum Structural basis of N6-adenosine methylation by the METTL3-METTL14 complex (2017), Nature, 542, 260 .

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Cloned(Commentary)

Cloned (Comment)	Organism
the METTL3-METTL14 complex is co-expressed in High Five (Trichoplusia ni) insect cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 18% (w/v) PEG 8000 and 0.1 M sodium citrate, pH 5.7	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D377A	the METTL3 mutation completely abolishes enzyme activity	Homo sapiens
D395A	the METTL3 mutation completely abolishes enzyme activity	Homo sapiens
E532A	the METTL3 mutation completely abolishes enzyme activity	Homo sapiens
H538A	the METTL3 mutation moderately reduces enzyme activity	Homo sapiens
N539A	the METTL3 mutation completely abolishes enzyme activity	Homo sapiens
N549A	the METTL3 mutation moderately reduces enzyme activity	Homo sapiens
Q550A	the METTL3 mutation moderately reduces enzyme activity	Homo sapiens
R536A	the METTL3 mutation moderately reduces enzyme activity	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + adenine in RNA	Homo sapiens	-	S-adenosyl-L-homocysteine + N6-methyladenine in RNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Source 15Q column chromatography, and Superdex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	in the enzyme complex, METTL3 primarily functions as the catalytic core, while METTL14 serves as an RNA-binding platform	Homo sapiens	?	-	-
additional information	no activity with RNA probe 5-GGGCUGGGCUGGGCUGGGCU-3	Homo sapiens	?	-	-
S-adenosyl-L-methionine + 5-GGACUGGACUGGACUGGACU-3	-	Homo sapiens	S-adenosyl-L-homocysteine + 5-GGm6ACUGGm6ACUGGm6ACUGGm6ACU-3	-	?
S-adenosyl-L-methionine + adenine in RNA	-	Homo sapiens	S-adenosyl-L-homocysteine + N6-methyladenine in RNA	-	?

Synonyms

Synonyms	Comment	Organism
m6A MTase	-	Homo sapiens
methyltransferase-like 14	-	Homo sapiens
methyltransferase-like 3	-	Homo sapiens
METTL14	-	Homo sapiens
METTL3	also known as MT-A70	Homo sapiens

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Release 2023.1 (January 2023)