Literature summary for 2.1.1.346 extracted from

Ruszkowska, A.; Ruszkowski, M.; Dauter, Z.; Brown, J.A.
Structural insights into the RNA methyltransferase domain of METTL16 (2018), Sci. Rep., 8, 5311 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop method at 19°C. Crystal structures of the N-terminal methyltransferase domain (residues 1-291) of human METTL16 (METTL16_291): an apo structure at 1.9 A resolution and a post-catalytic S-adenosylhomocysteine bound complex at 2.1 A resolution. The structures revealed a highly conserved Rossmann fold that is characteristic of class I S-adenosylmethionine-dependent methyltransferases and a large, positively charged groove. This groove likely represents the RNA-binding site and it includes structural elements unique to METTL16	Homo sapiens

Crystallization (Comment)

Organism

hanging-drop method at 19°C. Crystal structures of the N-terminal methyltransferase domain (residues 1-291) of human METTL16 (METTL16_291): an apo structure at 1.9 A resolution and a post-catalytic S-adenosylhomocysteine bound complex at 2.1 A resolution. The structures revealed a highly conserved Rossmann fold that is characteristic of class I S-adenosylmethionine-dependent methyltransferases and a large, positively charged groove. This groove likely represents the RNA-binding site and it includes structural elements unique to METTL16

Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + adenine in U6 snRNA	Homo sapiens	the enzyme methylates U6 spliceosomal RNA and interacts with the 3'-terminal RNA triple helix of MALAT1 (metastasis-associated lung adenocarcinoma transcript 1). Full-length METTL16 binds to the MALAT1 RNA triple helix, but monomeric METTL16_291 does not	S-adenosyl-L-homocysteine + N6-methyladenine in U6 snRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86W50	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + adenine in U6 snRNA	-	Homo sapiens	S-adenosyl-L-homocysteine + N6-methyladenine in U6 snRNA	-	?
S-adenosyl-L-methionine + adenine in U6 snRNA	the enzyme methylates U6 spliceosomal RNA and interacts with the 3'-terminal RNA triple helix of MALAT1 (metastasis-associated lung adenocarcinoma transcript 1). Full-length METTL16 binds to the MALAT1 RNA triple helix, but monomeric METTL16_291 does not	Homo sapiens	S-adenosyl-L-homocysteine + N6-methyladenine in U6 snRNA	-	?

Subunits

Subunits	Comment	Organism
dimer	full-length METTL16 forms a homodimer and METTL16_291 exists as a monomer	Homo sapiens
monomer	full-length METTL16 forms a homodimer and METTL16_291 exists as a monomer	Homo sapiens

Synonyms

Synonyms	Comment	Organism
m6A RNA methyltransferase	-	Homo sapiens
methyltransferase-like protein 16	-	Homo sapiens
METTL16	-	Homo sapiens