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Literature summary for 2.1.1.344 extracted from

  • Escobedo-Hinojosa, W.I.; Vences-Guzman, M.A.; Schubotz, F.; Sandoval-Calderon, M.; Summons, R.E.; Lopez-Lara, I.M.; Geiger, O.; Sohlenkamp, C.
    OlsG (Sinac_1600) Is an Ornithine Lipid N-Methyltransferase from the Planctomycete Singulisphaera acidiphila (2015), J. Biol. Chem., 290, 15102-15111 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.344

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100
-
 Singulisphaera acidiphila

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Singulisphaera acidiphila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0377
-
 an ornithine lipid at pH 8.0 and 28°C Singulisphaera acidiphila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + an ornithine lipid Singulisphaera acidiphila the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
 ?
S-adenosyl-L-methionine + an ornithine lipid Singulisphaera acidiphila DSM 18658T the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
 ?

Organism

Organism UniProt Comment Textmining
Singulisphaera acidiphila L0D9B6
-
-
Singulisphaera acidiphila DSM 18658T L0D9B6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + an ornithine lipid the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids Singulisphaera acidiphila S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
 ?
S-adenosyl-L-methionine + an ornithine lipid the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids Singulisphaera acidiphila DSM 18658T S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
 ?

Synonyms

Synonyms Comment Organism
OL N-methyltransferase
-
 Singulisphaera acidiphila
olsG gene name, ornithine lipid synthesis G Singulisphaera acidiphila
Sinac 1600
-
 Singulisphaera acidiphila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Singulisphaera acidiphila

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on, 1 mM used in assay conditions Singulisphaera acidiphila