Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Streptomyces davaonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0577 | - |
8-amino-8-demethyl-D-riboflavin | apparent value, at pH 8.0 and 37°C | Streptomyces davaonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | Streptomyces davaonensis | - |
S-adenosyl-L-homocysteine + roseoflavin | - |
? | |
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | Streptomyces davaonensis 768 | - |
S-adenosyl-L-homocysteine + roseoflavin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces davaonensis | E7BBN5 | - |
- |
Streptomyces davaonensis 768 | E7BBN5 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Streptomyces davaonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | - |
Streptomyces davaonensis | S-adenosyl-L-homocysteine + roseoflavin | - |
? | |
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | - |
Streptomyces davaonensis 768 | S-adenosyl-L-homocysteine + roseoflavin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Streptomyces davaonensis |
Synonyms | Comment | Organism |
---|---|---|
N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase | - |
Streptomyces davaonensis |
RosA | - |
Streptomyces davaonensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
8-amino-8-demethyl-D-riboflavin | at pH 8.0 and 37°C | Streptomyces davaonensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces davaonensis |