Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | dependent on | Sphingomonas paucimobilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingomonas paucimobilis |
gene ligM, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strains JM109 and BL21(DE3) | Sphingomonas paucimobilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of ligM leads to significant growth retardation on both vanillate and syringate, a ligM desA double mutant completely looses the ability to transform vanillate, phenotypes, overview | Sphingomonas paucimobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillate + tetrahydrofolate | Sphingomonas paucimobilis | - |
protocatechuate + 5-methyltetrahydrofolate | - |
? | |
vanillate + tetrahydrofolate | Sphingomonas paucimobilis | - |
protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway | ? | |
vanillate + tetrahydrofolate | Sphingomonas paucimobilis SYK-6 | - |
protocatechuate + 5-methyltetrahydrofolate | - |
? | |
vanillate + tetrahydrofolate | Sphingomonas paucimobilis SYK-6 | - |
protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | Q60FX1 | - |
- |
Sphingomonas paucimobilis | Q60FX1 | gene ligM | - |
Sphingomonas paucimobilis SYK-6 | Q60FX1 | - |
- |
Sphingomonas paucimobilis SYK-6 | Q60FX1 | gene ligM | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methylgallate + tetrahydrofolate | - |
Sphingomonas paucimobilis | gallate + 5-methyltetrahydrofolate | - |
? | |
additional information | syringate is no substrate | Sphingomonas paucimobilis | ? | - |
? | |
additional information | no substrate: syringate | Sphingomonas paucimobilis | ? | - |
? | |
additional information | syringate is no substrate | Sphingomonas paucimobilis SYK-6 | ? | - |
? | |
additional information | no substrate: syringate | Sphingomonas paucimobilis SYK-6 | ? | - |
? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis | protocatechuate + 5-methyltetrahydrofolate | - |
? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis | protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis | protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway | ? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis SYK-6 | protocatechuate + 5-methyltetrahydrofolate | - |
? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis SYK-6 | protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
vanillate + tetrahydrofolate | - |
Sphingomonas paucimobilis SYK-6 | protocatechuate + 5-methyltetrahydrofolate | procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway | ? |
Synonyms | Comment | Organism |
---|---|---|
LigM | - |
Sphingomonas paucimobilis |
tetrahydrofolate-dependent O-demethylase | - |
Sphingomonas paucimobilis |
vanillate/3-O-methylgallate O-demethylase | - |
Sphingomonas paucimobilis |
vanillate/3MGA O-demethylase | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Sphingomonas paucimobilis |
General Information | Comment | Organism |
---|---|---|
physiological function | disruption of LigM leads to significant growth retardation on both vanillate and syringate. The ability of the mutant to transform vanillate is markedly decreased, and it completely lost the 3-O-methylgallate O-demethylase activity. A LigM syringate demethylase DesA double mutant completely lost the ability to transform vanillate | Sphingomonas paucimobilis |