Application | Comment | Organism |
---|---|---|
analysis | reveals a fold typical of members of the SpoU clan of proteins, a subfamily of the alpha/beta-knot superfamily, with alpha-helical extensions at the N- and C-termini that are likely to be involved in tRNA binding | Aquifex aeolicus |
Cloned (Comment) | Organism |
---|---|
into vector pThioHisB and transformed into Escherichia coli strain DH5alpha, overexpressed in Escherichia coli Rosetta(DE3)/pLysS cells | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
by the hanging-drop method, at 1.85 A resolution, diffraction-quality crystals in the pH range 4.04.5, enzyme can not be crystallized with S-adenosyl-L-methionine or an analog in the active site | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
gel filtration | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67577 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, more than 99% pure | Aquifex aeolicus |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
TrmH | - |
Aquifex aeolicus |