Protein Variants | Comment | Organism |
---|---|---|
E124A | extensive structural alterations compared to wild-type, marked decrease in activity | Thermus thermophilus |
E124D | small increase in Km-value | Thermus thermophilus |
E124Q | no enzymic activity | Thermus thermophilus |
N152A | extensive structural alterations compared to wild-type, marked decrease in activity | Thermus thermophilus |
N152D | no enzymic activity | Thermus thermophilus |
N152E | no enzymic activity | Thermus thermophilus |
N35A | marked decrease in methyl transfer activity | Thermus thermophilus |
N35D | marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine | Thermus thermophilus |
N35Q | dramatic increase in Km-value | Thermus thermophilus |
P143Q | small increase in Km-value | Thermus thermophilus |
R41M | large increase in Km-value | Thermus thermophilus |
S150C | Km-value similar to wild-type, decrease in Vmax | Thermus thermophilus |
S150T | 4fold increase in Km-value | Thermus thermophilus |
S37G | 5fold increase in Km-value | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, wild-type | Thermus thermophilus | |
0.011 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant S150C | Thermus thermophilus | |
0.014 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant E124D | Thermus thermophilus | |
0.014 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant P143Q | Thermus thermophilus | |
0.037 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant S150T | Thermus thermophilus | |
0.051 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant S37G | Thermus thermophilus | |
0.083 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant N35A | Thermus thermophilus | |
0.67 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant N35D | Thermus thermophilus | |
0.92 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant R41M | Thermus thermophilus | |
2.1 | - |
S-adenosyl-L-methionine | pH 7.5, 50°C, mutant N35Q | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme hydroxylates guanosine at postion 18 in tRNA | Thermus thermophilus | ? | - |
? | |
S-adenosyl-L-methionine + yeast tRNA-Phe | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SpoU | - |
Thermus thermophilus |
TrmH | - |
Thermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus thermophilus | - |
- |
9.63 |