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Literature summary for 2.1.1.34 extracted from

  • Hori, H.; Suzuki, T.; Sugawara, K.; Inoue, Y.; Shibata, T.; Kuramitsu, S.; Yokoyama, S.; Oshima, T.; Watanabe, K.
    Identification and characterization of tRNA (Gm18) methyltransferase from Thermus thermophilus HB8: domain structure and conserved amino acid sequence motifs (2002), Genes Cells, 7, 259-272.
    View publication on PubMed
Search for more literature for 2.1.1.34

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with and without His-tag, amino acid sequence analysis and comparison Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
 guanosine18 in yeast tRNA-Phe
-
 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q9FAC4
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant from Escherichia coli Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA methylation site Thermus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
-
 ?

Synonyms

Synonyms Comment Organism
tRNA (Gm18) methyltransferase
-
 Thermus thermophilus