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Literature summary for 2.1.1.34 extracted from

  • Matsumoto, T.; Ohta, T.; Kumagai, I.; Oshima, T.; Murao, K.; Hasegawa, T.; Ishikura, H.; Watanabe, K.
    A thermostable Gm-methylase recognizes the tertiary structure of tRNA (1987), J. Biochem., 101, 1191-1198.
    View publication on PubMed
Search for more literature for 2.1.1.34

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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-
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Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
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-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA substrate recognition sites and mechanism Thermus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
-
 ?
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
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 optimal temperature for methylation is ca. 20°C lower than the melting temperature of the tRNA, overview Thermus thermophilus
45
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 Bacillus subtilis tRNALeu Thermus thermophilus
55
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 Bacillus subtilis tRNAThr Thermus thermophilus
60
-
 Bacillus subtilis tRNALys Thermus thermophilus
60
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 yeast tRNAPhe Thermus thermophilus
65
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 Bacillus subtilis tRNAVal Thermus thermophilus
65
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 Escherichia coli tRNAfMet Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 75 about half-maximal activity at 55°C and 75°C, Escherichia coli tRNAfMet Thermus thermophilus
55 68 about half-maximal activity at 55°C and 68°C, yeast tRNAPhe Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable below, tRNAPhe Thermus thermophilus
70
-
 stable below, tRNAMet Thermus thermophilus