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Literature summary for 2.1.1.34 extracted from

  • Matsumoto, T.; Watanabe, K.; Ohta, T.
    Recognition mechanism of tRNA with tRNA(guanosine-2)methyltransferase from Thermus thermophilus HB 27 (1984), Nucleic Acids Symp. Ser., 15, 131-134.
No PubMed abstract available
Search for more literature for 2.1.1.34

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA substrate recognition sites and mechanism Thermus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
-
 ?
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 yeast tRNAPhe Thermus thermophilus
65
-
 Escherichia coli tRNAfMet Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
-
 Thermus thermophilus