Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
(S)-reticuline | pH 7.0, 30°C | Papaver somniferum | |
0.085 | - |
(R)-reticuline | pH 7.0, 30°C | Papaver somniferum | |
0.168 | - |
S-adenosyl-L-methionine | pH 7.0, 30°C | Papaver somniferum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41900 | - |
- |
Papaver somniferum |
45000 | - |
- |
Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Papaver somniferum | RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations | ? | - |
? | |
S-adenosyl-L-methionine + (+)-bulbocapnine | Papaver somniferum | 75% substrate conversion | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (+)-glaucine | Papaver somniferum | 47% substrate conversion | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R)-reticuline | Papaver somniferum | 98% substrate conversion | S-adenosyl-L-homocysteine + (S)-tembetarine | - |
? | |
S-adenosyl-L-methionine + (S)-corytuberine | Papaver somniferum | 82% substrate conversion | S-adenosyl-L-homocysteine + magnoflorine | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | Papaver somniferum | 47% substrate conversion | S-adenosyl-L-homocysteine + (S)-tembetarine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | A0A1C9U5X5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | highest transcript level | Papaver somniferum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations | Papaver somniferum | ? | - |
? | |
additional information | RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations | Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (+)-bulbocapnine | 75% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (+)-glaucine | 47% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R)-reticuline | 98% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-tembetarine | - |
? | |
S-adenosyl-L-methionine + (R,S)-tetrahydropapaverine | 24% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (S)-corytuberine | 82% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + magnoflorine | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | 47% substrate conversion | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-tembetarine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41900, calculated, x * 45000, SDS-PAGE | Papaver somniferum |
Synonyms | Comment | Organism |
---|---|---|
NMT3 | - |
Papaver somniferum |
RNMT | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Papaver somniferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
(S)-reticuline | pH 7.0, 30°C | Papaver somniferum | |
0.047 | - |
S-adenosyl-L-methionine | pH 7.0, 30°C | Papaver somniferum | |
0.053 | - |
(R)-reticuline | pH 7.0, 30°C | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | substrate (S)-reticuline | Papaver somniferum |
8.5 | - |
substrate (R)-reticuline | Papaver somniferum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Papaver somniferum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Papaver somniferum | calculated | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | suppression of the gene encoding reticuline N-methyltransferase results in a significant decrease in magnoflorine accumulation and a concomitant increase in corytuberine levels in roots | Papaver somniferum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
S-adenosyl-L-methionine | pH 7.0, 30°C | Papaver somniferum | |
0.62 | - |
(R)-reticuline | pH 7.0, 30°C | Papaver somniferum | |
0.65 | - |
(S)-reticuline | pH 7.0, 30°C | Papaver somniferum |