BRENDA - Enzyme Database
show all sequences of 2.1.1.333

Crystal structures of BchU, a methyltransferase involved in bacteriochlorophyll c biosynthesis, and its complex with S-adenosylhomocysteine: implications for reaction mechanism

Wada, K.; Yamaguchi, H.; Harada, J.; Niimi, K.; Osumi, S.; Saga, Y.; Oh-Oka, H.; Tamiaki, H.; Fukuyama, K.; J. Mol. Biol. 360, 839-849 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli B834(DE3) cells
Chlorobaculum tepidum
Crystallization (Commentary)
Crystallization (Commentary)
Organism
apoenzyme and in complex with S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl (pH 8.5), 12% (v/v) glycerol
Chlorobaculum tepidum
Engineering
Protein Variants
Commentary
Organism
H150A
the mutant loses 96.3% of the wild type activity
Chlorobaculum tepidum
H290A
the mutant loses 99.2% of the wild type activity
Chlorobaculum tepidum
Y246F
the mutant loses 98.2% of the wild type activity
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
Chlorobaculum tepidum ATCC49652
-
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Chlorobaculum tepidum
Q8KGE0
-
-
Chlorobaculum tepidum ATCC49652
Q8KGE0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
-
740864
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
-
ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
-
740864
Chlorobaculum tepidum ATCC49652
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
-
ir
Subunits
Subunits
Commentary
Organism
homodimer
-
Chlorobaculum tepidum
Synonyms
Synonyms
Commentary
Organism
bchU
-
Chlorobaculum tepidum
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli B834(DE3) cells
Chlorobaculum tepidum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apoenzyme and in complex with S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl (pH 8.5), 12% (v/v) glycerol
Chlorobaculum tepidum
Engineering (protein specific)
Protein Variants
Commentary
Organism
H150A
the mutant loses 96.3% of the wild type activity
Chlorobaculum tepidum
H290A
the mutant loses 99.2% of the wild type activity
Chlorobaculum tepidum
Y246F
the mutant loses 98.2% of the wild type activity
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
Chlorobaculum tepidum ATCC49652
-
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
ir
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
-
740864
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
-
ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide
-
740864
Chlorobaculum tepidum ATCC49652
S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
-
Chlorobaculum tepidum
Other publictions for EC 2.1.1.333
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741105
Tsukatani
Bacteriochlorophyll homolog co ...
Chlorobaculum limnaeum
Photochem. Photobiol. Sci.
12
2195-2201
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740864
Wada
Crystal structures of BchU, a ...
Chlorobaculum tepidum, Chlorobaculum tepidum ATCC49652
J. Mol. Biol.
360
839-849
2006
-
-
1
1
3
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739800
Harada
Crystallization and preliminar ...
Chlorobaculum tepidum
Acta Crystallogr. Sect. F
61
712-714
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740445
Harada
In vitro activity of C-20 meth ...
Chlorobaculum tepidum
FEBS Lett.
579
1983-1987
2005
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740604
Maresca
The bchU gene of Chlorobium te ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
J. Bacteriol.
186
2558-2566
2004
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-