Literature summary for 2.1.1.333 extracted from

Wada, K.; Yamaguchi, H.; Harada, J.; Niimi, K.; Osumi, S.; Saga, Y.; Oh-Oka, H.; Tamiaki, H.; Fukuyama, K.
Crystal structures of BchU, a methyltransferase involved in bacteriochlorophyll c biosynthesis, and its complex with S-adenosylhomocysteine: implications for reaction mechanism (2006), J. Mol. Biol., 360, 839-849.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834(DE3) cells	Chlorobaculum tepidum

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme and in complex with S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl (pH 8.5), 12% (v/v) glycerol	Chlorobaculum tepidum

Protein Variants

Protein Variants	Comment	Organism
H150A	the mutant loses 96.3% of the wild type activity	Chlorobaculum tepidum
H290A	the mutant loses 99.2% of the wild type activity	Chlorobaculum tepidum
Y246F	the mutant loses 98.2% of the wild type activity	Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide	Chlorobaculum tepidum	-	S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c	-	ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide	Chlorobaculum tepidum ATCC49652	-	S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c	-	ir

Organism

Organism	UniProt	Comment	Textmining
Chlorobaculum tepidum	Q8KGE0	-	-
Chlorobaculum tepidum ATCC49652	Q8KGE0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide	-	Chlorobaculum tepidum	S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c	-	ir
S-adenosyl-L-methionine + 8-ethyl-12-methyl-bacteriochlorophyllide	-	Chlorobaculum tepidum ATCC49652	S-adenosyl-L-homocysteine + 8-ethyl-12-methylbacteriochlorophyllide c	-	ir

Subunits

Subunits	Comment	Organism
homodimer	-	Chlorobaculum tepidum

Synonyms

Synonyms	Comment	Organism
bchU	-	Chlorobaculum tepidum

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Chlorobaculum tepidum

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Release 2023.1 (January 2023)