BRENDA - Enzyme Database
show all sequences of 2.1.1.333

In vitro activity of C-20 methyltransferase, BchU, involved in bacteriochlorophyll c biosynthetic pathway in green sulfur bacteria

Harada, J.; Saga, Y.; Yaeda, Y.; Oh-Oka, H.; Tamiaki, H.; FEBS Lett. 579, 1983-1987 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + zinc bacteriopheophorbide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + zinc bacteriopheophorbide c
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Chlorobaculum tepidum
Q8KGE0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
metal-free bacteriopheophorbide d cannot be methylated by the enzyme
740445
Chlorobaculum tepidum
?
-
-
-
-
S-adenosyl-L-methionine + zinc bacteriopheophorbide d
-
740445
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + zinc bacteriopheophorbide c
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Chlorobaculum tepidum
Synonyms
Synonyms
Commentary
Organism
bchU
-
Chlorobaculum tepidum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + zinc bacteriopheophorbide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + zinc bacteriopheophorbide c
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
metal-free bacteriopheophorbide d cannot be methylated by the enzyme
740445
Chlorobaculum tepidum
?
-
-
-
-
S-adenosyl-L-methionine + zinc bacteriopheophorbide d
-
740445
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + zinc bacteriopheophorbide c
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Chlorobaculum tepidum
Other publictions for EC 2.1.1.333
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741105
Tsukatani
Bacteriochlorophyll homolog co ...
Chlorobaculum limnaeum
Photochem. Photobiol. Sci.
12
2195-2201
2013
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1
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1
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1
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740864
Wada
Crystal structures of BchU, a ...
Chlorobaculum tepidum, Chlorobaculum tepidum ATCC49652
J. Mol. Biol.
360
839-849
2006
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1
1
3
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2
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2
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2
1
1
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1
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1
1
1
3
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2
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2
1
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739800
Harada
Crystallization and preliminar ...
Chlorobaculum tepidum
Acta Crystallogr. Sect. F
61
712-714
2005
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1
1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
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1
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-
740445
Harada
In vitro activity of C-20 meth ...
Chlorobaculum tepidum
FEBS Lett.
579
1983-1987
2005
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
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1
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2
1
1
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1
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1
1
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1
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2
1
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740604
Maresca
The bchU gene of Chlorobium te ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
J. Bacteriol.
186
2558-2566
2004
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2
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9
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2
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2
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2
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2
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