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show all sequences of 2.1.1.333

Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis

Harada, J.; Wada, K.; Yamaguchi, H.; Oh-oka, H.; Tamiaki, H.; Fukuyama, K.; Acta Crystallogr. Sect. F 61, 712-714 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl pH 8.5, and 12% (v/v) glycerol
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a bacteriochlorophyllide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlorobaculum tepidum
Q8KGE0
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-affinity resin column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a bacteriochlorophyllide d
-
739800
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl pH 8.5, and 12% (v/v) glycerol
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a bacteriochlorophyllide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-affinity resin column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a bacteriochlorophyllide d
-
739800
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
-
?
Other publictions for EC 2.1.1.333
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741105
Tsukatani
Bacteriochlorophyll homolog co ...
Chlorobaculum limnaeum
Photochem. Photobiol. Sci.
12
2195-2201
2013
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740864
Wada
Crystal structures of BchU, a ...
Chlorobaculum tepidum, Chlorobaculum tepidum ATCC49652
J. Mol. Biol.
360
839-849
2006
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3
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3
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1
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739800
Harada
Crystallization and preliminar ...
Chlorobaculum tepidum
Acta Crystallogr. Sect. F
61
712-714
2005
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1
1
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740445
Harada
In vitro activity of C-20 meth ...
Chlorobaculum tepidum
FEBS Lett.
579
1983-1987
2005
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1
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1
1
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5
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1
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1
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740604
Maresca
The bchU gene of Chlorobium te ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
J. Bacteriol.
186
2558-2566
2004
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9
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