Cloned (Comment) | Organism |
---|---|
coexpression of both METTL1 and WDR4 in Saccharomyces cerevisiae | Homo sapiens |
ORF YDL201w encodes Trm8 and ORF YDR165w encodes Trm82, both proteins form a complex with m7G-methyltransferase activity, Trm8 contains a binding domain for S-adenosyl-L-methionine: GXGXG, coexpression of Trm8/Trm82 in Escherichia coli BL21 Codon Plus (DE3) as His6-fusion proteins under control of the Ptac-promoter | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | strains lacking either Trm8 or Trm82 yield extracts with no detectable enzyme activity, and have severely reduced m7G-modified tRNA in vivo | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | Saccharomyces cerevisiae | complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
strains BY4743, 33523, 33899 and YM317 | - |
Purification (Comment) | Organism |
---|---|
copurification of 2 proteins, Trm8 and Trm82 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, but Trm82 is not an absolute requirement for activity in vitro, Trm8 may be the catalytically active subunit | Saccharomyces cerevisiae | additional information | - |
? | |
additional information | proteins METTL1 and WDR4 are required for m7G-methyltransferase activity | Homo sapiens | additional information | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptors: tRNACys, tRNATrp, tRNAPro, tRNAMet, tRNAMet-i and 2 of each tRNAPhe, tRNAVal and tRNALys, methylation at G46 in the extra loop | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptor: yeast pre-tRNAPhe | Homo sapiens | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptor: yeast pre-tRNAPhe | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Homo sapiens |
37 | - |
30fold higher activity when assayed at 37°C than at 30°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |