Literature summary for 2.1.1.33 extracted from

Paolella, G.; Ciliberto, G.; Traboni, C.; Cimino, F.; Salvatore, F.
Effect of adenosylhomocysteine and other analog thioethers on a prokaryotic tRNA (guanine-7)-methyltransferase (1982), Arch. Biochem. Biophys., 219, 149-154.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Methylthioinosine	activates at 0.05-0.2 mM	Salmonella enterica subsp. enterica serovar Typhimurium
S-Inosylhomocysteine	activates at 0.05-0.2 mM	Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability	Organism
freezing inactivates	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
methylthioadenosine	competitive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
Methylthioinosine	weak competitive inhibitor, above 0.5 mM	Salmonella enterica subsp. enterica serovar Typhimurium
additional information	not inhibited by butylthioadenosine, thioethanoladenosine, isobutylthioadenosine	Salmonella enterica subsp. enterica serovar Typhimurium
S-Adenosyl-D-homocysteine	competitive inhibitor, L-enantiomer more potent than D-enantiomer by 2 orders of magnitude	Salmonella enterica subsp. enterica serovar Typhimurium
S-adenosyl-DL-homocysteine	competitive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
S-adenosyl-L-cysteine	competitive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
S-adenosyl-L-homocysteine	potent competitive product inhibition, L-enantiomer more potent than D-enantiomer by 2 orders of magnitude	Salmonella enterica subsp. enterica serovar Typhimurium
S-Inosylhomocysteine	weak competitive inhibitor, above 0.5 mM	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine46 in tRNA	Salmonella enterica subsp. enterica serovar Typhimurium	specific enzyme involved in the maturation of tRNA	S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	wild-type strain TA 265	-

Purification (Commentary)

Purification (Comment)	Organism
partial purification by phosphocellulose column	Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	mechanism	Salmonella enterica subsp. enterica serovar Typhimurium	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine46 in tRNA	methyl group acceptor: undermethylated tRNA from Salmonella typhimurium CS 14, NH2- and COOH-group of S-adenosyl-L-methionine as well as the length of hydrocarbon chain connecting them to the aromatic ring of adenosine, chirality at the alpha-C atom and NH2-group of adenosine are important for substrate binding to enzyme	Salmonella enterica subsp. enterica serovar Typhimurium	S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	-	?
S-adenosyl-L-methionine + guanine46 in tRNA	specific enzyme involved in the maturation of tRNA	Salmonella enterica subsp. enterica serovar Typhimurium	S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	-	?

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.008	-	S-adenosyl-L-homocysteine	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.064	-	S-adenosyl-DLhomocysteine	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.46	-	methylthioadenosine	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.55	-	S-adenosyl-L-cysteine	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.84	-	S-Adenosyl-D-homocysteine	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)