Cloned (Comment) | Organism |
---|---|
SD_1168 from Shewanella denitrificans is cloned into pET-30a(+)for protein overexpression. SD_1168 is found exclusively in inclusion bodies when overexpressed in either Escherichia coli or Streptomyces lividans. All efforts to express soluble recombinant protein are unsuccessful. Therefore SD_1168 is refolded using an established refolding procedure | Shewanella denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S] cluster | the enzyme binds 6.1 mol of iron per mol protein and 4.0 mol of sulfid | Shewanella denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64800 | - |
x * 64800, SDS-PAGE | Shewanella denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella denitrificans | - |
- |
- |
Shewanella denitrificans OS217 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + demethylphosphinothricin + reduced acceptor | - |
Shewanella denitrificans | S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + phosphinothricin + oxidized acceptor | - |
? | |
2 S-adenosyl-L-methionine + demethylphosphinothricin + reduced acceptor | - |
Shewanella denitrificans OS217 | S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + phosphinothricin + oxidized acceptor | - |
? | |
2 S-adenosyl-L-methionine + N-acetyldemethylphosphinothricin + reduced acceptor | - |
Shewanella denitrificans | S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + N-acetylphosphinothricin + oxidized acceptor | - |
? | |
2 S-adenosyl-L-methionine + N-acetyldemethylphosphinothricin + reduced acceptor | - |
Shewanella denitrificans OS217 | S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + N-acetylphosphinothricin + oxidized acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64800, SDS-PAGE | Shewanella denitrificans |
Synonyms | Comment | Organism |
---|---|---|
SD_1168 | - |
Shewanella denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
methylcob(III)alamin | the enzyme catalyzes P-methylation using radical S-adenosyl-L-methionine-dependent chemistry with cobalamin as a coenzymemethyl donor of the reaction. The enzyme is active in the absence of methylcobalamin if the strong reductant titanium(III)citrate and hydroxocobalamin are provided | Shewanella denitrificans |