Any feedback?
Literature summary for 2.1.1.325 extracted from
- Aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone III, exhibits substrate control (2014), J. Insect Physiol., 64, 62-73.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli One Shot BL21 (DE3) cells | Aedes aegypti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by EDTA | Aedes aegypti | |
| Zn2+ | 16fold inhibition at 10 mM | Aedes aegypti |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000008 | - |
(trans/cis/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00016 | - |
juvenile hormone III acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00028 | - |
juvenile hormone I acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00223 | - |
(2E,6E)-farnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00387 | - |
(cis/trans/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.01027 | - |
(cis/trans/trans)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.01468 | - |
juvenile hormone III acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.01997 | - |
homofarnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions are not required for enzyme activity | Aedes aegypti |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Aedes aegypti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + (2E,6E)-farnesoate | Aedes aegypti | - |
S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate | - |
? | |
| S-adenosyl-L-methionine + juvenile hormone III acid | Aedes aegypti | - |
S-adenosyl-L-homocysteine + juvenile hormone III | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aedes aegypti | Q206L4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Aedes aegypti |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corpora allata | - |
Aedes aegypti | - |
| ovary | - |
Aedes aegypti | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + (2E,6E)-farnesoate | - |
Aedes aegypti | S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate | - |
? | |
| S-adenosyl-L-methionine + (cis/trans/cis)-juvenile hormone I acid | - |
Aedes aegypti | ? | - |
? | |
| S-adenosyl-L-methionine + (cis/trans/trans)-juvenile hormone I acid | - |
Aedes aegypti | ? | - |
? | |
| S-adenosyl-L-methionine + (trans/cis/cis)-juvenile hormone I acid | - |
Aedes aegypti | ? | - |
? | |
| S-adenosyl-L-methionine + homofarnesoate | - |
Aedes aegypti | S-adenosyl-L-homocysteine + methylhomofarnesoate | - |
? | |
| S-adenosyl-L-methionine + juvenile hormone I acid bisepoxide | - |
Aedes aegypti | ? | - |
? | |
| S-adenosyl-L-methionine + juvenile hormone III acid | - |
Aedes aegypti | S-adenosyl-L-homocysteine + juvenile hormone III | - |
? | |
| S-adenosyl-L-methionine + juvenile hormone III acid bisepoxide | - |
Aedes aegypti | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Aedes aegypti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| JHAMT | - |
Aedes aegypti |
| juvenile hormone acid methyl transferase | - |
Aedes aegypti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000006 | - |
(trans/cis/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00001 | - |
juvenile hormone III acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.000011 | - |
juvenile hormone I acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.00016 | - |
homofarnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.000217 | - |
(2E,6E)-farnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.000245 | - |
(cis/trans/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.000875 | - |
(cis/trans/trans)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.001148 | - |
juvenile hormone III acid | at pH 7.9 and 25°C | Aedes aegypti | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00803 | - |
homofarnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.03929 | - |
juvenile hormone I acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.06146 | - |
juvenile hormone III acid bisepoxide | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.06335 | - |
(cis/trans/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.0782 | - |
juvenile hormone III acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.08523 | - |
(cis/trans/trans)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.09716 | - |
(2E,6E)-farnesoate | at pH 7.9 and 25°C | Aedes aegypti | |
| 0.7708 | - |
(trans/cis/cis)-juvenile hormone I acid | at pH 7.9 and 25°C | Aedes aegypti |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
