Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharopolyspora spinosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM used in assay conditions | Saccharopolyspora spinosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Saccharopolyspora spinosa |
28730 | - |
2 * 28730, calculated from amino acid sequence | Saccharopolyspora spinosa |
61200 | - |
gel filtration | Saccharopolyspora spinosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose | Saccharopolyspora spinosa | - |
S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora spinosa | Q9ALP0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl S-200 gel filtration | Saccharopolyspora spinosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose | - |
Saccharopolyspora spinosa | S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28000, SDS-PAGE | Saccharopolyspora spinosa |
homodimer | 2 * 28730, calculated from amino acid sequence | Saccharopolyspora spinosa |
Synonyms | Comment | Organism |
---|---|---|
SpnS | - |
Saccharopolyspora spinosa |