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Literature summary for 2.1.1.316 extracted from

  • Grüschow, S.; Chang, L.C.; Mao, Y.; Sherman, D.H.
    Hydroxyquinone O-methylation in mitomycin biosynthesis (2007), J. Am. Chem. Soc., 129, 6470-6476.
    View publication on PubMed
Search for more literature for 2.1.1.316

Activating Compound

Activating Compound Comment Organism Structure
EDTA presence of 1 mM EDTA results in a slight but significantly higher activity Streptomyces lavendulae

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged MmcR is expressed in Escherichia coli BL21(DE3) Streptomyces lavendulae

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ completely inhibits the methylation reaction Streptomyces lavendulae
Fe2+ 4-fold decrease of enzyme activity Streptomyces lavendulae
Mn2+ 4-fold decrease of enzyme activity Streptomyces lavendulae
additional information increasing the salt concentration from 50 to 500 mM resuls in a gradual decrease in activity to 60% of the initial value Streptomyces lavendulae
Zn2+ completely inhibits the methylation reaction Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 S-adenosyl-L-methionine pH 7.4, 30°C Streptomyces lavendulae
0.17
-
 7-demethylmitomycin A pH 7.4, 30°C Streptomyces lavendulae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ or Ca2+ do not alter the reactivity of the enzyme Streptomyces lavendulae

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae Q9X5T6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 6-demethylmitomycin A the enzyme catalyzes the 7-O-methylation of both C9alpha- and C9beta-configured 7-hydroxymitomycins in vitro Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin A
-
 ?
S-adenosyl-L-methionine + 6-demethylmitomycin B the enzyme catalyzes the 7-O-methylation of both C9alpha- and C9beta-configured 7-hydroxymitomycins in vitro Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin B
-
 ?
S-adenosyl-L-methionine + 7-demethylmitomycin A
-
 Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin A
-
 ?
S-adenosyl-L-methionine + 7-demethylmitomycin A
-
 Streptomyces lavendulae ?
-
 r

Synonyms

Synonyms Comment Organism
MmcR
-
 Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 7-demethylmitomycin A pH 7.4, 30°C Streptomyces lavendulae
0.0018
-
 S-adenosyl-L-methionine pH 7.4, 30°C Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Streptomyces lavendulae
7.4
-
 assay at Streptomyces lavendulae

General Information

General Information Comment Organism
malfunction an engineered Streptomyces lavendulae strain in which the mmcR methyltransferase gene has been deleted fails to produce the three mitomycins A, B, and C Streptomyces lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000458
-
 7-demethylmitomycin B pH 7.4, 30°C Streptomyces lavendulae
0.01
-
 7-demethylmitomycin A pH 7.4, 30°C Streptomyces lavendulae