Activating Compound | Comment | Organism | Structure |
---|---|---|---|
TRMT112 | a multifunctional methyltransferase subunit TRM112-like protein with methyltransferase activity, it participates both in methylation of protein and tRNA species. TRMT112 is required for WBSCR22 metabolic stability | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme depletion by siRNA treatment | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | secondary structure of the 18S rRNA, overview | ? | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | Homo sapiens | m7G1639 methylation | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43709 | WBS22; gene WBSCR22 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | secondary structure of the 18S rRNA, overview | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | m7G1639 methylation | Homo sapiens | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
WBSCR22 | - |
Homo sapiens |
WBSCR22-TRMT112 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | a multifunctional methyltransferase subunit TRM112-like protein with methyltransferase activity participates both in methylation of protein and tRNA species. TRMT112 is required for WBSCR22 metabolic stability | Homo sapiens | |
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | WBSCR22-TRMT112 is the functional homologue of Saccharomyces cerevisiae Bud23-Trm112 | Homo sapiens |
malfunction | HeLa cells depleted of WBSCR22 show a mild reduction in 30S, indicative of reduced cleavage at site 2, and significant accumulation of 18S-E revealing inhibition of cleavage at site 3. Long and short truncated forms of 18S precursors are detected. On WBSCR22 depletion, the amount of mature 18S rRNA is markedly reduced and the 28S/18S rRNA ratio consequently increased. TRMT112 depletion leads to processing phenotypes largely similar to those observed upon WBSCR22 depletion (moderate 30S reduction and accumulation of 18S-E). The processing defects do not depend on p53 and are essentially the same, with minor differences, in different cell types. Kinetics of the pre-rRNA processing defects in HeLa cells by in vivo metabolic labeling, phenotype, overview | Homo sapiens |
metabolism | DIMT1L, WBSCR22, and TRMT112 are required for distinct pre-rRNA processing steps, and the pre-rRNA processing defects are conserved in different cell types and do not depend on p53 | Homo sapiens |
physiological function | the enzyme is required for distinct pre-rRNA processing reactions leading to synthesis of 18S rRNA. In human cells riboosome biogenesis requires the presence of the modification enzyme rather than its RNA-modifying catalytic activity. Methylation activity of WBSCR22-TRMT112 is not necessary for pre-rRNA processing | Homo sapiens |