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Literature summary for 2.1.1.309 extracted from

  • Zorbas, C.; Nicolas, E.; Wacheul, L.; Huvelle, E.; Heurgue-Hamard, V.; Lafontaine, D.L.
    The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis (2015), Mol. Biol. Cell, 26, 2080-2095.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
TRMT112 a multifunctional methyltransferase subunit TRM112-like protein with methyltransferase activity, it participates both in methylation of protein and tRNA species. TRMT112 is required for WBSCR22 metabolic stability Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme depletion by siRNA treatment Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens secondary structure of the 18S rRNA, overview ?
-
?
S-adenosyl-L-methionine + guanine10 in tRNA Homo sapiens m7G1639 methylation S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43709 WBS22; gene WBSCR22
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information secondary structure of the 18S rRNA, overview Homo sapiens ?
-
?
S-adenosyl-L-methionine + guanine10 in tRNA m7G1639 methylation Homo sapiens S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
?

Synonyms

Synonyms Comment Organism
WBSCR22
-
Homo sapiens
WBSCR22-TRMT112
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information a multifunctional methyltransferase subunit TRM112-like protein with methyltransferase activity participates both in methylation of protein and tRNA species. TRMT112 is required for WBSCR22 metabolic stability Homo sapiens
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
evolution WBSCR22-TRMT112 is the functional homologue of Saccharomyces cerevisiae Bud23-Trm112 Homo sapiens
malfunction HeLa cells depleted of WBSCR22 show a mild reduction in 30S, indicative of reduced cleavage at site 2, and significant accumulation of 18S-E revealing inhibition of cleavage at site 3. Long and short truncated forms of 18S precursors are detected. On WBSCR22 depletion, the amount of mature 18S rRNA is markedly reduced and the 28S/18S rRNA ratio conse­quently increased. TRMT112 depletion leads to processing phenotypes largely similar to those observed upon WB­SCR22 depletion (moderate 30S reduction and accumulation of 18S-E). The processing defects do not depend on p53 and are essentially the same, with minor differences, in different cell types. Kinetics of the pre-rRNA processing defects in HeLa cells by in vivo metabolic labeling, phenotype, overview Homo sapiens
metabolism DIMT1L, WBSCR22, and TRMT112 are required for distinct pre-rRNA processing steps, and the pre-rRNA processing defects are conserved in different cell types and do not depend on p53 Homo sapiens
physiological function the enzyme is required for distinct pre-rRNA processing reactions leading to synthesis of 18S rRNA. In human cells riboosome biogenesis requires the presence of the modification enzyme rather than its RNA-modifying catalytic activity. Methylation activity of WBSCR22-TRMT112 is not necessary for pre-rRNA processing Homo sapiens