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Literature summary for 2.1.1.309 extracted from

  • Figaro, S.; Wacheul, L.; Schillewaert, S.; Graille, M.; Huvelle, E.; Mongeard, R.; Zorbas, C.; Lafontaine, D.L.; Heurgue-Hamard, V.
    Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575 (2012), Mol. Cell. Biol., 32, 2254-2267.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
TRM112 Trm112 is a small zinc finger protein of 15 kDa which acts as a coactivator of several class I S-adenosyl-L-methionine (SAM)-dependent MTases. Trm112 interacts directly with Bud23 in vitro and that it is absolutely required for Bud23 stability in vivo. Trm112 increases the solubility of Bud23 Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
recombinant co-expression of His6-tagged Bud23 with untagged Trm112 in Escherichia coli Saccharomyces cerevisiae

General Stability

General Stability Organism
Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo ?
-
?
S-adenosyl-L-methionine + guanine1575 in 18S rRNA Saccharomyces cerevisiae
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S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
tandem purification of recombinant His6-tagged Bud23 with untagged Trm112 by nickel affinity chromatography from Escherichia coli, Trm112 increases the solubility of Bud23 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + guanine1575 in 18S rRNA
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA
-
?

Synonyms

Synonyms Comment Organism
18S rRNA methyltransferase
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Saccharomyces cerevisiae
BUD23
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
evolution Bud23 is a class I S-adenosyl-L-methionine (SAM)-dependent MTase Saccharomyces cerevisiae
malfunction trm112DELTA cells are deficient for Bud23-mediated 18S rRNA methylation at position G1575 and for small ribosome subunit formation. Bud23 failure to bind nascent preribosomes activates a nucleolar surveillance pathway involving the TRAMP complexes, leading to preribosome degradation Saccharomyces cerevisiae
metabolism Bud23 failure to bind nascent preribosomes activates a nucleolar surveillance pathway involving the TRAMP complexes, leading to preribosome degradation Saccharomyces cerevisiae