Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli Rosetta 2 (DE3) pLysS cells as a hexahistidine-tagged protein. The recombinant proteins are found exclusively in inclusion bodies. After refolding and concentration, the protein is reconstituted with additional iron and sulfide to maximize cluster formation | Streptomyces wedmorensis |
Protein Variants | Comment | Organism |
---|---|---|
C282A | the mutant enzyme is not able to produce the catalytic [4Fe-4S]+1 cluster | Streptomyces wedmorensis |
C282A/C286A/C289A | the triple-variant is very unstable and precipitated during purification and subsequent manipulation for experiments. It completely lacks the [4Fe-4S]+1 cluster | Streptomyces wedmorensis |
C286A | the mutant enzyme is not able to produce the catalytic [4Fe-4S]+1 cluster | Streptomyces wedmorensis |
C289A | the mutant enzyme is not able to produce the catalytic [4Fe-4S]+1 cluster | Streptomyces wedmorensis |
additional information | site-directed mutagenesis of the cysteine residues in the radical SAM CxxxCxxC motif indicates that each residue is essential for functional cluster formation | Streptomyces wedmorensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S] cluster | the [4Fe-4S] cluster undergoes a transition between a +2 resting state and a +1 active state. Site-directed mutagenesis of the cysteine residues in the radical SAM CxxxCxxC motif indicates that each residue is essential for functional cluster formation | Streptomyces wedmorensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60400 | - |
10 * or 12 * 60400, SDS-PAGE | Streptomyces wedmorensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + methylcob(III)alamin + 2-hydroxyethylphosphonate | Streptomyces wedmorensis | the enzyme is involved in the biosynthesis of the broad-spectrum antibiotic fosfomycin | 5'-deoxyadenosine + L-methionine + cob(III)alamin + (2S)-2-hydroxypropylphosphonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces wedmorensis | Q56184 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces wedmorensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + methylcob(III)alamin + 2-hydroxyethylphosphonate | - |
Streptomyces wedmorensis | 5'-deoxyadenosine + L-methionine + cob(III)alamin + (2S)-2-hydroxypropylphosphonate | - |
? | |
S-adenosyl-L-methionine + methylcob(III)alamin + 2-hydroxyethylphosphonate | the enzyme is involved in the biosynthesis of the broad-spectrum antibiotic fosfomycin | Streptomyces wedmorensis | 5'-deoxyadenosine + L-methionine + cob(III)alamin + (2S)-2-hydroxypropylphosphonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | 10 * or 12 * 60400, SDS-PAGE | Streptomyces wedmorensis |
Synonyms | Comment | Organism |
---|---|---|
fom3 | - |
Streptomyces wedmorensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | - |
Streptomyces wedmorensis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of the broad-spectrum antibiotic fosfomycin | Streptomyces wedmorensis |