BRENDA - Enzyme Database show
show all sequences of 2.1.1.301

Catalytic promiscuity of a bacterial alpha-N-methyltransferase

Zhang, Q.; van der Donk, W.A.; FEBS Lett. 586, 3391-3397 (2012)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
Streptomyces sp.
-
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
Streptomyces sp. OH-4156
-
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. OH-4156
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + haloduracin
-
729730
Streptomyces sp.
?
-
-
-
?
S-adenosyl-L-methionine + haloduracin
-
729730
Streptomyces sp. OH-4156
?
-
-
-
?
S-adenosyl-L-methionine + L-Lys
the enzyme can also methylate the epsilon-amino group of L-Lys. Peptides with Lys in the first or second position from the N-terminus (entries 9-10) are not modified by the enzyme, but a peptide with a Lys at the C-terminus (entry 11) is methylated, with both di- and tetra-methylated products detected in the reaction mixture
729730
Streptomyces sp.
S-adenosyl-L-homocysteine + N6-methyl-L-lysine
-
-
-
?
S-adenosyl-L-methionine + L-Lys
the enzyme can also methylate the epsilon-amino group of L-Lys. Peptides with Lys in the first or second position from the N-terminus (entries 9-10) are not modified by the enzyme, but a peptide with a Lys at the C-terminus (entry 11) is methylated, with both di- and tetra-methylated products detected in the reaction mixture
729730
Streptomyces sp. OH-4156
S-adenosyl-L-homocysteine + N6-methyl-L-lysine
-
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
-
729730
Streptomyces sp.
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
-
729730
Streptomyces sp. OH-4156
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
-
?
S-adenosyl-L-methionine + nisin
-
729730
Streptomyces sp.
?
-
-
-
?
S-adenosyl-L-methionine + nisin
-
729730
Streptomyces sp. OH-4156
?
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
Streptomyces sp.
-
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
Streptomyces sp. OH-4156
-
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + haloduracin
-
729730
Streptomyces sp.
?
-
-
-
?
S-adenosyl-L-methionine + haloduracin
-
729730
Streptomyces sp. OH-4156
?
-
-
-
?
S-adenosyl-L-methionine + L-Lys
the enzyme can also methylate the epsilon-amino group of L-Lys. Peptides with Lys in the first or second position from the N-terminus (entries 9-10) are not modified by the enzyme, but a peptide with a Lys at the C-terminus (entry 11) is methylated, with both di- and tetra-methylated products detected in the reaction mixture
729730
Streptomyces sp.
S-adenosyl-L-homocysteine + N6-methyl-L-lysine
-
-
-
?
S-adenosyl-L-methionine + L-Lys
the enzyme can also methylate the epsilon-amino group of L-Lys. Peptides with Lys in the first or second position from the N-terminus (entries 9-10) are not modified by the enzyme, but a peptide with a Lys at the C-terminus (entry 11) is methylated, with both di- and tetra-methylated products detected in the reaction mixture
729730
Streptomyces sp. OH-4156
S-adenosyl-L-homocysteine + N6-methyl-L-lysine
-
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
-
729730
Streptomyces sp.
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
-
?
S-adenosyl-L-methionine + N-terminal L-alanine-[cypemycin]
-
729730
Streptomyces sp. OH-4156
S-adenosyl-L-homocysteine + N-terminal N,N-dimethyl-L-alanine-[cypemycin]
-
-
-
?
S-adenosyl-L-methionine + nisin
-
729730
Streptomyces sp.
?
-
-
-
?
S-adenosyl-L-methionine + nisin
-
729730
Streptomyces sp. OH-4156
?
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of the peptide antibiotic cypemycin
Streptomyces sp.
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of the peptide antibiotic cypemycin
Streptomyces sp.
Other publictions for EC 2.1.1.301
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729730
Zhang
Catalytic promiscuity of a bac ...
Streptomyces sp., Streptomyces sp. OH-4156
FEBS Lett.
586
3391-3397
2012
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1
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730805
Claesen
Genome mining and genetic anal ...
Streptomyces sp., Streptomyces sp. OH-4156
Proc. Natl. Acad. Sci. USA
107
16297-16302
2010
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