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Literature summary for 2.1.1.296 extracted from

  • Arhin, G.K.; Li, H.; Ullu, E.; Tschudi, C.
    A protein related to the vaccinia virus cap-specific methyltransferase VP39 is involved in cap 4 modification in Trypanosoma brucei (2006), RNA, 12, 53-62.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.296

Cloned(Commentary)

Cloned (Comment) Organism
the mt57-/- mutant cells are complemented with the triple MT57 mutant W62D/G63D/Q64D and with MT57 containing the K266A mutation, both mutants do not reestablish the +5 primer extension stop to wild-type levels Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
K266A site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells Trypanosoma brucei
additional information genetic ablation of MT57 is compatible with cell viability and leads to the accumulation of SL RNA with a cap structure defective at positions +3 and +4.. Transsplicing utilization of the SL RNA is not detectably affected in mt57-/- cells, analysis of the SL cap structure in mt57-/- cells, overview Trypanosoma brucei
W62D/G63D/Q64D site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Trypanosoma brucei 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38DJ3
-
-

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
MT57
-
 Trypanosoma brucei
TbMT57
-
 Trypanosoma brucei

General Information

General Information Comment Organism
evolution MT57 homologs are only found in trypanosomatid protozoa that have a cap 4 structure and in poxviruses, of which vaccinia virus is a prototype, TbMT48 or TbMT57 are two protein components of the cap 4 biosynthetic machinery Trypanosoma brucei
malfunction downregulation by RNAi or genetic ablation of TbMT57 result in the accumulation of SL RNA missing 2'-O-methyl groups at positions +3 and +4 and thus bearing a cap 2 rather than a cap 4. Genetic ablation of MT57 results in viable cells with no apparent defect in SL RNA transsplicing, suggesting that MT57 is not essential or that trypanosomes have developed alternate mechanisms to counteract the absence of this protein Trypanosoma brucei
physiological function the enzyme is involved in formation of the cap 4 structure, a cap structure of the SL RNA unique in eukaryotes with 4 nucleotides after the cap carrying a total of seven methyl groups. Modifications at the +3 and +4 positions are important for binding to the nuclear cap-binding complex, but MT57 is not essential. The Trypanosoma brucei cap binding complex can distinguish between a cap 4 and an m7G structure and it has a much higher affinity for the cap 4 substrate Trypanosoma brucei