Cloned (Comment) | Organism |
---|---|
gene ftmD, encoded in the ftm gene cluster, recombinant expression in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineered production of tryprostatins in Escherichia coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp., i.e. four genes, ftmABCD, on two compatible plasmids | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 6-hydroxytryprostatin B | Aspergillus fumigatus | - |
S-adenosyl-L-homocysteine + tryprostatin A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WAW6 | Af293 R202L variant; isolates Af293, A1163 and BM939, gene ftmD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 6-hydroxytryprostatin B | - |
Aspergillus fumigatus | S-adenosyl-L-homocysteine + tryprostatin A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ftmD | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
evolution | all ftm genes are expressed almost equally well in both Af293 and BM939 strains. But a point mutation found in ftmD in the genome of Af293 causes an arginine to leucine substitution at position 202 of FtmD, resulting in a dramatic decrease of the catalytic efficiency of FtmD. This mutated form of FtmD appeared not functioning under physiological conditions in Af293 to produce any detectable levels of TPS-A or any downstream metabolites | Aspergillus fumigatus |