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Literature summary for 2.1.1.292 extracted from

  • Connors, N.C.; Strohl, W.R.
    Partial purification and properties of carminomycin 4-0-methyltransferase from Streptomyces sp. strain C5 (1993), J. Gen. Microbiol., 139, 1353-1362.
No PubMed abstract available
Search for more literature for 2.1.1.292

Inhibitors

Inhibitors Comment Organism Structure
adenine 0.25 mM, 8% inhibition Streptomyces sp.
additional information homocysteine, adenosine, epsilon-rhodomycinone, daunomycin, and daunomycinone show little or no inhibitory activity Streptomyces sp.
S-adenosyl-L-homocysteine 0.025 mM, 87% inhibition Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 carminomycin pH 8.0, 37°C Streptomyces sp.
0.001
-
 13-dihydrocarminomycin pH 8.0, 37°C Streptomyces sp.
0.025
-
 S-adenosyl-L-methionine pH 8.0, 37°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cation requirement can be demonstrated for the partially purified enzyme Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE Streptomyces sp.
166000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 13-dihydrocarminomycin Streptomyces sp. terminal enzyme in the daunomycin biosynthesis pathway S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
S-adenosyl-L-methionine + carminomycin Streptomyces sp.
-
 S-adenosyl-L-homocysteine + daunorubicin
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0194
-
 pH 8.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified enzyme does not catalyse methylation of the aglycones carminomycinone or 13-dihydrocarminomycinone Streptomyces sp. ?
-
 ?
S-adenosyl-L-methionine + 13-dihydrocarminomycin terminal enzyme in the daunomycin biosynthesis pathway Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
S-adenosyl-L-methionine + 13-dihydrocarminomycin the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicinol
-
 ?
S-adenosyl-L-methionine + carminomycin
-
 Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicin
-
 ?
S-adenosyl-L-methionine + carminomycin the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin Streptomyces sp. S-adenosyl-L-homocysteine + daunorubicin
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
DnrK
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 90% of maximal activity, 45°C: about 90% of maximal activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8 pH 7.5: 40% of maximal activity, pH 8.0: optimum. No pH values above pH 8.0 are evaluated due to the inherent instability of S-adenosyl-L-methionine at alkaline pH Streptomyces sp.